Department of Advanced Medicine in Biotechnology and Robotics, Graduate School of Medicine, Nagoya University, Nagoya 461-0047, Japan.
Biomaterials. 2012 Mar;33(7):2177-86. doi: 10.1016/j.biomaterials.2011.12.009. Epub 2011 Dec 20.
Adipose tissue-derived stem cell (ASC) transplantation, when used in combination with heparin, has proven to be an effective treatment for acute liver failure in mice. However, the behavior and organ-specific accumulation of transplanted ASCs alone or in combination with heparin is poorly understood. In this paper, we investigated whether quantum dots (QDs) labeling using octa-arginine peptide (R8) for ASCs could be applied for in vivo fluorescence imaging in mice with acute liver failure, and analyzed the behavior and organ-specific accumulation of ASCs that were transplanted alone or in combination with heparin using an IVIS(®) Spectrum analysis. Almost all of the transplanted ASCs were observed to accumulate in the lungs within 10 min without heparin. However, when heparin was used in combination with the ASCs, the accumulation of the transplanted ASCs was found not only in the lungs but also in the liver. The region of interest (ROI) analysis of ex vivo fluorescence imaging showed that the accumulation rate of transplanted ASCs in the liver increased to about 30%. In the time course analysis, the accumulation rate of ASCs in the liver was about 10% in 1 day and was maintained at that level for at least 2 day. We observed that heparin was effective for increasing the accumulation of transplanted ASCs in the liver using fluorescence imaging technology. We suggest that fluorescence imaging by means of QDs labeling using R8 can be useful for tracing the transplanted cells.
脂肪组织来源的干细胞（ASC）移植与肝素联合使用已被证明可有效治疗小鼠急性肝衰竭。然而，单独或联合肝素使用时，移植 ASC 的行为和器官特异性积累仍知之甚少。在本文中，我们研究了使用八聚精氨酸肽（R8）对 ASC 进行量子点（QD）标记是否可用于急性肝衰竭小鼠的体内荧光成像，并使用 IVIS（®）Spectrum 分析分析了单独或联合肝素移植的 ASC 的行为和器官特异性积累。在没有肝素的情况下，几乎所有移植的 ASC 都在 10 分钟内观察到在肺部聚集。然而，当肝素与 ASC 联合使用时，不仅在肺部，而且在肝脏中也发现了移植的 ASC 的积累。离体荧光成像的 ROI 分析表明，移植的 ASC 在肝脏中的积累率增加到约 30%。在时间过程分析中，移植的 ASC 在肝脏中的积累率在 1 天内约为 10%，并至少维持在该水平 2 天。我们观察到荧光成像技术中肝素可有效增加移植 ASC 在肝脏中的积累。我们认为，使用 R8 进行 QD 标记的荧光成像可用于追踪移植细胞。
