Zhang Xiaorong, He Xiaoxiao, Wang Kemin, Wang Yonghong, Li Huimin, Tan Weihong
State Key Laboratory of Chemo/Biosensing and Chemometrics, Biomedical Engineering Center, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China.
J Nanosci Nanotechnol. 2009 Oct;9(10):5738-44. doi: 10.1166/jnn.2009.1287.
The unique optoelectronic and physicochemical properties of gold nanoparticles are significantly dependent on the particle size, shape and structure. In this paper, biosynthesis of size-controlled gold nanoparticles using fungus Penicillium sp. is reported. Fungus Penicillium sp. could successfully bioreduce and nucleate AuCl4(-) ions, and lead to the assembly and formation of intracellular Au nanoparticles with spherical morphology and good monodispersity after exposure to HAuCl4 solution. Reaction temperature, as an important physiological parameter for fungus Penicillium sp. growth, could significantly control the size of the biosynthesized Au nanoparticles. The biological compositions and FTIR spectra analysis of fungus Penicillium sp. exposed to HAuCl4 solution indicated the intracellular reducing sugar played an important role in the occurrence of intracellular reduction of AuCl4(-) ions and the growth of gold nanoparticles. Furthermore, the intracellular gold nanoparticles could be easily separated from the fungal cell lysate by ultrasonication and centrifugation.
金纳米粒子独特的光电和物理化学性质显著依赖于颗粒大小、形状和结构。本文报道了利用青霉菌合成尺寸可控的金纳米粒子。青霉菌能够成功地对AuCl4(-)离子进行生物还原和成核，并在暴露于HAuCl4溶液后导致细胞内形成具有球形形态且单分散性良好的金纳米粒子的组装和形成。反应温度作为青霉菌生长的一个重要生理参数，能够显著控制生物合成的金纳米粒子的尺寸。对暴露于HAuCl4溶液的青霉菌进行的生物成分和傅里叶变换红外光谱分析表明，细胞内还原糖在AuCl4(-)离子的细胞内还原以及金纳米粒子的生长过程中发挥了重要作用。此外，通过超声处理和离心，细胞内金纳米粒子能够很容易地从真菌细胞裂解物中分离出来。
