State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, Hunan University, Key Laboratory for Bio-Nanotechnology and Molecule Engineering of Hunan Province, Changsha 410082, P R. China.
J Biomed Nanotechnol. 2011 Apr;7(2):245-54. doi: 10.1166/jbn.2011.1285.
In this paper, the intracellular gold nanoparticles were biosynthesized using three fungi including Aureobasidium pullulans (A. pullulans), Fusarium sp. and Fusarium oxysporum (F. oxysporum) after immersion the fungal cells in AuCl4- ions solution. UV-vis and FTIR spectrum, and biochemical compositions analysis of Au nano-fungal cells suggested that active biomolecules of reducing sugar of A. pullulans, and proteins in Fusarium sp. and F. oxysporum were tested positive of providing the function of the reduction of AuCI4- ions and the formation of the gold crystals. SDS-PAGE analysis of purified protein from gold nanoparticles synthesized by three fungi showed that three proteins with molecular weight (WM) about 100 kDa, 25 kDa and 19 kDa were in the gold nanoparticles by Fusarium sp. and two proteins with WM about 25 kDa and 19 kDa were in gold nanoparticles of F oxysporum. Further, three purified fungal proteins with WM about 100 kDa, 25 kDa and 19 kDa from gold nanoparticles by Fusarium sp. identified by LC-MS/MS, named plasma membrane ATPase, 3-glucan binding protein and glyceraldehyde-3-phosphate dehydrogenase, respectively. The Au nano-fungal cells ultrathin sections of Fusarium sp. and F. oxysporum showed that the gold nanoparticles mainly produced in intracellular vacuoles of fungal cells. The growth of gold nanoparticles in three fungal cells indicated the reducing sugar led to the gold nanoparticles in spherical morphology and proteins benefited to the gold aggregates.
本文采用三种真菌（出芽短梗霉（A. pullulans）、镰刀菌属（Fusarium sp.）和尖孢镰刀菌（F. oxysporum）），将真菌细胞浸泡在 AuCl4-离子溶液中，生物合成了细胞内金纳米颗粒。通过紫外-可见光谱和傅里叶变换红外光谱以及生化成分分析表明，出芽短梗霉的还原糖和镰刀菌属及尖孢镰刀菌中的蛋白质等活性生物分子，被证实具有还原 AuCI4-离子和形成金晶体的功能。对三种真菌合成的金纳米颗粒进行纯化蛋白的 SDS-PAGE 分析表明，在镰刀菌属的金纳米颗粒中存在三种分子量（WM）约为 100 kDa、25 kDa 和 19 kDa 的蛋白质，在尖孢镰刀菌的金纳米颗粒中存在两种分子量（WM）约为 25 kDa 和 19 kDa 的蛋白质。此外，通过 LC-MS/MS 鉴定出三种从镰刀菌属的金纳米颗粒中纯化得到的具有 WM 约 100 kDa、25 kDa 和 19 kDa 的真菌蛋白，分别命名为质膜 ATP 酶、3-葡聚糖结合蛋白和甘油醛-3-磷酸脱氢酶。对镰刀菌属和尖孢镰刀菌属的 Au 纳米真菌细胞进行超薄切片，结果表明金纳米颗粒主要产生在真菌细胞的细胞内液泡中。金纳米颗粒在三种真菌细胞中的生长表明，还原糖导致金纳米颗粒呈球形形态，而蛋白质则有利于金纳米颗粒的聚集。
