Department of Oral and Maxillofacial Surgery, University Hospital Aachen, Aachen, Germany.
Head Face Med. 2009 Nov 12;5:23. doi: 10.1186/1746-160X-5-23.
Cellular reactions to alloplastic bone substitute materials (BSM) are a subject of interest in basic research. In regenerative dentistry, these bone grafting materials are routinely combined with enamel matrix derivatives (EMD) in order to additionally enhance tissue regeneration.
The aim of this study was to evaluate the proliferative activity of human osteogenic cells after incubation over a period of seven days with commercial BSM of various origin and chemical composition. Special focus was placed on the potential additional benefit of EMD on cellular proliferation.
Except for PerioGlas, osteogenic cell proliferation was significantly promoted by the investigated BSM. The application of EMD alone also resulted in significantly increased cellular proliferation. However, a combination of BSM and EMD resulted in only a moderate additional enhancement of osteogenic cell proliferation.
The application of most BSM, as well as the exclusive application of EMD demonstrated a positive impact on the proliferation of human osteogenic cells in vitro. In order to increase the benefit from substrate combination (BSM + EMD), further studies on the interactions between BSM and EMD are needed.
细胞对异质骨替代材料（BSM）的反应是基础研究的一个关注领域。在再生牙科中，这些骨移植材料通常与釉基质衍生物（EMD）联合使用，以额外增强组织再生。
本研究的目的是评估在七天的孵育期内，各种来源和化学成分的商业 BSM 对人成骨细胞增殖活性的影响。特别关注 EMD 对细胞增殖的潜在额外益处。
除了 PerioGlas，研究中的 BSM 均显著促进了成骨细胞的增殖。单独应用 EMD 也导致细胞增殖显著增加。然而，BSM 和 EMD 的联合应用仅导致成骨细胞增殖适度增加。
大多数 BSM 的应用以及 EMD 的单独应用均显示对人成骨细胞体外增殖有积极影响。为了增加基质组合（BSM+EMD）的益处，需要进一步研究 BSM 和 EMD 之间的相互作用。
