Dipartimento di Psichiatria, Neurobiologia, Farmacologia e Biotecnologie, University of Pisa, Pisa, Italy.
Psychiatry Res. 2010 Jan 30;175(1-2):54-7. doi: 10.1016/j.psychres.2009.03.009. Epub 2009 Nov 11.
The aim of our study was to investigate and compare the dopamine (DA) transporter (DAT) in resting lymphocytes of 20 psychotic patients and 20 healthy control subjects, by means of both the binding parameters (Bmax and Kd) of 3H-WIN 35,428, and the reuptake parameters (Vmax and Km) of 3H-DA. The results showed that both the Bmax of 3H-WIN 35,428 binding and the Vmax of 3H-DA reuptake of the patients were significantly lower than those of healthy subjects, while the Kd or Km did not show any change. These findings, while indicating a reduced density of the lymphocyte DAT proteins, provide further support of the role of DA in psychoses and suggest that DA alterations may not be limited to brain structures.
我们的研究目的是通过 3H-WIN 35,428 的结合参数（Bmax 和 Kd）和 3H-DA 的摄取参数（Vmax 和 Km），来检测和比较 20 名精神病患者和 20 名健康对照者静息淋巴细胞中的多巴胺（DA）转运体（DAT）。结果表明，患者的 3H-WIN 35,428 结合的 Bmax 和 3H-DA 摄取的 Vmax 均明显低于健康对照组，而 Kd 或 Km 则没有变化。这些发现表明，淋巴细胞 DAT 蛋白的密度降低，进一步支持了 DA 在精神病中的作用，并表明 DA 的改变可能不仅局限于大脑结构。
