Ko M K, Lee W R, McKechnie N M, Hall-Parker B
Department of Ophthalmology, University of Glasgow, Western Infirmary.
Br J Ophthalmol. 1991 Jan;75(1):54-60. doi: 10.1136/bjo.75.1.54.
Light microscopy (including fluorescence microscopy) and electron microscopy were applied to a study of the photoreceptor-retinal pigment epithelium (RPE) complex in a human eye which had been severely traumatised nine months prior to enucleation. The main feature of interest was a massive accumulation of lipofuscin in the retinal pigment epithelium at the posterior pole, and quantitative fluorescence microscopy provided values three times those obtained in appropriate control tissue. The photoreceptor layer was normal at the posterior pole but became progressively atrophic towards the periphery. The concentration of lipopofuscin was proportional to the degree of preservation of the retinal photoreceptors. By electron microscopy the cells in the RPE were seen to be packed with a mixture of lipofuscin granules and melanolysosomal complexes, but occasional photoreceptor phagosomes were found. Bruch's membrane and the choriocapillaris were normal. We attribute this hitherto unreported abnormality of the RPE after trauma to a dysfunction consequent on an overload of the monolayer by photoreceptor debris at the time of trauma.
光学显微镜（包括荧光显微镜）和电子显微镜被用于研究一只在摘除眼球前九个月受到严重创伤的人眼的光感受器 - 视网膜色素上皮（RPE）复合体。主要关注的特征是在眼球后极的视网膜色素上皮中大量脂褐素积累，定量荧光显微镜检查得到的值是在适当对照组织中获得值的三倍。后极处的光感受器层正常，但向周边逐渐萎缩。脂褐素的浓度与视网膜光感受器的保存程度成正比。通过电子显微镜观察，RPE中的细胞充满了脂褐素颗粒和黑素溶酶体复合体的混合物，但偶尔也发现有光感受器吞噬体。布鲁赫膜和脉络膜毛细血管正常。我们将这种创伤后RPE迄今未报道的异常归因于创伤时光感受器碎片使单层超负荷导致的功能障碍。
