Mezache Nadjet, Derbré Séverine, Akkal Salah, Laouer Hocine, Séraphin Denis, Richomme Pascal
Université Mentouri, Constantine, laboratoire de Phytochimie et d'Analyses Physico-chimiques et Biologiques, département de Chimie, Faculté des Sciences exactes, Algérie.
Nat Prod Commun. 2009 Oct;4(10):1357-62.
Phenolic derivatives such as quinones, acid-phenols and flavonoids were successfully isolated from a n-butanolic fraction of Senecio giganteus Desf. (Asteraceae) flowers, namely jacaranone (1), 3a-hydroxy-3,3a,7,7a-tetrahydrobenzofuran-2,6-dione (2), chlorogenic acid (3), hyperoside (4), quercetin 3-O-beta-D-robinobioside (5), isorhamnetin-3-O-beta-D-glucuronide (6), quercetin-3-O-beta-D-glucuronide (7), and isorhamnetin-3-O-beta-D-glucuronide-6"-methyl ester (8). These compounds were purified through either classical polyamide filtration followed by fractionation on Si gel, or through fast centrifugal partition chromatography (FCPC). Using FCPC, the major compounds could be readily isolated from the crude n-butanolic fraction. Compounds 1-8 were identified by means of spectroscopic and spectrometric analysis (UV, 1H, 13C and 2D NMR, and MS). This work described for the first time the phytochemical composition of this endemic Algerian plant.
从巨千里光（菊科）花朵的正丁醇提取物中成功分离出了酚类衍生物，如醌类、酸酚类和黄酮类化合物，即紫铆茵酮（1）、3a-羟基-3,3a,7,7a-四氢苯并呋喃-2,6-二酮（2）、绿原酸（3）、金丝桃苷（4）、槲皮素3-O-β-D-刺槐二糖苷（5）、异鼠李素-3-O-β-D-葡萄糖醛酸苷（6）、槲皮素-3-O-β-D-葡萄糖醛酸苷（7）和异鼠李素-3-O-β-D-葡萄糖醛酸苷-6”-甲酯（8）。这些化合物通过经典的聚酰胺过滤，然后在硅胶上进行分馏，或通过快速离心分配色谱法（FCPC）进行纯化。使用FCPC，可以很容易地从粗正丁醇提取物中分离出主要化合物。通过光谱和光谱分析（紫外、1H、13C和2D NMR以及质谱）鉴定了化合物1-8。这项工作首次描述了这种阿尔及利亚特有植物的植物化学成分。
