Centre for Geographic Medicine Research (Coast), Kenya Medical Research Institute/Wellcome Trust Research Programme, Kilifi, Kenya.
Transfusion. 2010 Mar;50(3):611-6. doi: 10.1111/j.1537-2995.2009.02489.x. Epub 2009 Nov 13.
In sub-Saharan Africa umbilical cord blood may be a useful source of blood for transfusion. Before clinical trials, evidence is needed that cord blood donations, which vary greatly in volume, can be collected and stored into a fixed volume of anticoagulant-preservative solution obviating the need for prestorage processing.
Twenty-four umbilical cord whole blood (UC-WB) donations were collected into 21 mL of CPDA-1 and refrigerated for 35 days. The Kenya Blood Transfusion Service provided 12 adult-donated whole blood (AD-WB) controls. Supernatant hemoglobin (Hb) and potassium were assayed at 7-day intervals.
UC-WB red blood cell hemolysis and potassium loss increased throughout storage but did not differ significantly with cord blood volume. Hemolysis rates did not differ significantly between UC-WB and AD-WB but UC-WB potassium loss was slightly but significantly greater than AD-WB on Days 2, 7, and 14 (p < 0.05). In the AD-WB controls, eight were low volume (<405 mL), two had total Hb of less than 45 g, and two showed hemolysis greater than 0.8% by Day 28.
Variable volumes of UC-WB can be stored for 35 days without prestorage processing and further work into its suitability for transfusion to children is justified. The quality of conventional AD-WB is a concern and needs further evaluation.
在撒哈拉以南非洲，脐带血可能是输血的一种有用来源。在进行临床试验之前，需要有证据表明，脐带血捐献量差异很大，可以采集并储存在固定体积的抗凝保存溶液中，从而避免了储存前处理的需要。
将 24 份脐带全血（UC-WB）采集到 21 毫升 CPDA-1 中并冷藏 35 天。肯尼亚血液服务中心提供了 12 份成人捐献全血（AD-WB）对照。每隔 7 天检测上清液中的血红蛋白（Hb）和钾含量。
UC-WB 红细胞溶血和钾丢失在整个储存过程中逐渐增加，但与脐带血体积无显著差异。UC-WB 的溶血率与 AD-WB 无显著差异，但 UC-WB 的钾丢失在第 2、7 和 14 天略高于 AD-WB（p<0.05）。在 AD-WB 对照组中，有 8 份为低体积（<405 毫升），有 2 份总 Hb 低于 45 克，有 2 份在第 28 天出现溶血率大于 0.8%。
可以在不进行储存前处理的情况下储存 35 天的可变体积 UC-WB，进一步研究其对儿童输血的适用性是合理的。常规 AD-WB 的质量令人担忧，需要进一步评估。
