Fondren Orthopedic Group, 7401 S Main Street, Houston, TX 77030-4509, USA.
Am J Infect Control. 2010 Apr;38(3):199-204. doi: 10.1016/j.ajic.2009.07.006. Epub 2009 Nov 12.
Prevention of postsurgical infection is preferable to treatment. Prevention requires identification and control of the potential sources of microbial contamination. This study investigated whether the density of airborne particulates can predict the density of viable airborne bacteria at the surgery site.
A standard particle analyzer was used to measure the number and diameter of airborne particulates during 22 joint arthroplasty surgeries. An impact air sampler and standard culture plates were used to identify and count colony-forming units (CFU).
Particulate density averaged >500,000 particles/m(3) per 10-minute interval, and 1786 CFU were identified, primarily gram-positive cocci. A particle density > or = 10 microm explained 41% of the variation in CFU density. Particle and CFU density increased with longer surgery duration and higher staff counts.
These findings support the use of environmental controls that isolate and protect the surgical site from airborne particulates and contamination.
预防术后感染优于治疗。预防需要识别和控制微生物污染的潜在来源。本研究旨在调查空气中颗粒物的密度是否可以预测手术部位空气中存活细菌的密度。
在 22 例关节置换手术中，使用标准粒子分析仪测量空气中颗粒物的数量和直径。使用冲击式空气采样器和标准培养平板来鉴定和计数集落形成单位（CFU）。
颗粒物密度平均为每 10 分钟间隔>500,000 个颗粒/立方米，鉴定出 1786 个 CFU，主要为革兰阳性球菌。粒径>或=10μm 可解释 CFU 密度变化的 41%。颗粒物和 CFU 密度随手术时间延长和工作人员数量增加而增加。
这些发现支持使用环境控制措施，将手术部位与空气中的颗粒物和污染隔离开来并加以保护。
