一种反式作用核糖开关控制单核细胞增生李斯特菌中毒力调节因子PrfA的表达。
A trans-acting riboswitch controls expression of the virulence regulator PrfA in Listeria monocytogenes.
作者信息
Loh Edmund, Dussurget Olivier, Gripenland Jonas, Vaitkevicius Karolis, Tiensuu Teresa, Mandin Pierre, Repoila Francis, Buchrieser Carmen, Cossart Pascale, Johansson Jörgen
机构信息
Department of Molecular Biology, Umeå University, 90187 Umeå, Sweden.
出版信息
Cell. 2009 Nov 13;139(4):770-9. doi: 10.1016/j.cell.2009.08.046.
Riboswitches are RNA elements acting in cis, controlling expression of their downstream genes through a metabolite-induced alteration of their secondary structure. Here, we demonstrate that two S-adenosylmethionine (SAM) riboswitches, SreA and SreB, can also function in trans and act as noncoding RNAs in Listeria monocytogenes. SreA and SreB control expression of the virulence regulator PrfA by binding to the 5'-untranslated region of its mRNA. Absence of the SAM riboswitches SreA and SreB increases the level of PrfA and virulence gene expression in L. monocytogenes. Thus, the impact of the SAM riboswitches on PrfA expression highlights a link between bacterial virulence and nutrient availability. Together, our results uncover an unexpected role for riboswitches and a distinct class of regulatory noncoding RNAs in bacteria.
核糖开关是一种顺式作用的RNA元件，通过代谢物诱导其二级结构的改变来控制下游基因的表达。在此，我们证明了两种S-腺苷甲硫氨酸（SAM）核糖开关SreA和SreB也能反式作用，并在单核细胞增生李斯特菌中作为非编码RNA发挥作用。SreA和SreB通过与毒力调节因子PrfA的mRNA的5'-非翻译区结合来控制其表达。SAM核糖开关SreA和SreB的缺失会增加单核细胞增生李斯特菌中PrfA的水平和毒力基因的表达。因此，SAM核糖开关对PrfA表达的影响突出了细菌毒力与营养可用性之间的联系。总之，我们的结果揭示了核糖开关和一类独特的细菌调节性非编码RNA的意外作用。
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