Cell & Molecular Biology Laboratory, Asan Institute for Life Sciences, Asan Medical Center, 388-1 PoongNap-Dong, SongPa-Gu, Seoul 138-736, Republic of Korea.
Biomaterials. 2010 Feb;31(6):1213-8. doi: 10.1016/j.biomaterials.2009.10.057. Epub 2009 Nov 14.
We investigated the combination of human adipose tissue derived stem cells (ADSC) and in vivo gel-forming methoxy poly (ethyleneglycol)-poly (epsilon-caprolactone) (MPEG-PCL) as a muscle regeneration matrix, with and without inclusion of vascular endothelial cell growth factor (VEGF). VEGF(165)-treated stem cell grafts showed significant proliferation and differentiation into muscle tissue in vivo. Importantly, the inclusion of VEGF enhanced vascularization. This scaffold supported preconditioned ADSC, and allowed them to differentiate into mature muscle tissues in vivo, indicating that ADSC of human origin and MPEG-PCL scaffolds provided an appropriate environment for cellular growth and expansion. Our results thus provide a potential solution to the major obstacle encountered in the engineering of thick complex tissues, which require an adequate blood supply to maintain cell viability during tissue growth and to induce appropriate structural organization. Therefore, the combination of ADSC and in vivo gel-forming MPEG-PCL with VEGF(165) might serve as a suitable non-invasive biomaterial for clinical muscle regeneration applications.
我们研究了人脂肪组织来源的干细胞（ADSC）与体内形成凝胶的甲氧基聚（乙二醇）-聚（ε-己内酯）（MPEG-PCL）的组合，以及是否包含血管内皮生长因子（VEGF）。VEGF（165）处理的干细胞移植物在体内显示出显著的增殖和向肌肉组织分化。重要的是，包含 VEGF 可增强血管生成。这种支架支持预处理的 ADSC，并允许它们在体内分化为成熟的肌肉组织，表明人源 ADSC 和 MPEG-PCL 支架为细胞生长和扩增提供了合适的环境。因此，ADSC 与体内形成凝胶的 MPEG-PCL 与 VEGF（165）的组合可能成为临床肌肉再生应用中一种合适的非侵入性生物材料。
