Department of Internal Medicine, Faculty of Medicine, Saga University, 5-1-1 Nabeshima, Saga 849-8501, Japan.
Lung Cancer. 2010 Aug;69(2):232-8. doi: 10.1016/j.lungcan.2009.10.010. Epub 2009 Nov 14.
Mina53, a novel target gene product of c-Myc, is overexpressed in various malignancies. We previously demonstrated that Mina53 is overexpressed in lung cancer patients from the early clinical stages. In this paper, the association between disease prognosis and Mina53 expression in lung cancer patients is analyzed; we found that overexpression of Mina53 in lung cancer patients is associated with favorable prognosis. Statistical analysis using the Kaplan-Meier method showed that patients with negative staining for Mina53 had significantly shorter survival than patients with positive staining for Mina53, especially in stage I or with squamous cell carcinoma. Because the major cause of death in lung cancer patients after surgery is distant metastasis, the effect on cancer cell invasiveness was analyzed for the mechanisms involved in the association with favorable outcome. Overexpression of Mina53 in H226B, a lung squamous cell carcinoma cell line, inhibited cancer cell invasion. Transfection with mina53 shRNA increased the number of invading cells. These results suggest that Mina53 immunostaining is a useful prognostic marker--especially in the early stage of lung cancer--and that Mina53 negative patients should be managed particularly carefully after surgery.
Mina53 是 c-Myc 的一种新型靶基因产物，在各种恶性肿瘤中过表达。我们之前的研究表明，Mina53 在肺癌患者的早期临床阶段过表达。在本文中，我们分析了 Mina53 在肺癌患者中的表达与疾病预后之间的关系，发现肺癌患者 Mina53 的过表达与良好的预后相关。Kaplan-Meier 统计分析表明，Mina53 阴性染色的患者的生存时间明显短于 Mina53 阳性染色的患者，尤其是在 I 期或鳞状细胞癌患者中。由于肺癌患者手术后死亡的主要原因是远处转移，因此分析了 Mina53 过表达与良好预后相关的机制中涉及的对癌细胞侵袭性的影响。在肺鳞状细胞癌细胞系 H226B 中过表达 Mina53 抑制了癌细胞的侵袭。转染 mina53 shRNA 增加了侵袭细胞的数量。这些结果表明，Mina53 免疫染色是一种有用的预后标志物，尤其是在肺癌的早期阶段，Mina53 阴性的患者在手术后应特别小心地进行管理。
