Nagano Naoki, Taoka Yousuke, Honda Daisuke, Hayashi Masahiro
Department of Biological Production and Environmental Science, Faculty of Agriculture, University of Miyazaki, Miyazaki 889-2192, JAPAN.
J Oleo Sci. 2009;58(12):623-8. doi: 10.5650/jos.58.623.
The effects of carbon sources, seawater concentration and seawater component in a culture medium were investigated to optimize culture conditions for growth by a marine thraustochytrid Aurantiochytrium limacinum strain mh0186. Strain mh0186 could utilize D-glucose, D-fructose and D-mannose as carbon sources. Seawater concentrations between 12.5 - 200% were required for good growth, and a single omission of magnesium sulfate from the seawater reduced the growth of the cells. Jar fermentor trials were carried out for the purpose of docosahexaenoic acid (DHA) production by strain mh0186. The total fatty acid content of the cell was 466.5 mg/g dry cells, and biomass and DHA yield were estimated as 23.1 g/L and 4.3 g/L, respectively, at 26 h. The daily production of DHA by the strain was 4.0 g/L/d, suggesting that the higher DHA production rate of our strain mh0186 should be appropriate for industrial production of DHA.
研究了培养基中碳源、海水浓度和海水成分对海洋破囊壶菌 Aurantiochytrium limacinum 菌株 mh0186 生长的影响，以优化其培养条件。菌株 mh0186 能够利用 D-葡萄糖、D-果糖和 D-甘露糖作为碳源。良好生长需要 12.5%-200%的海水浓度，海水中单独省略硫酸镁会降低细胞生长。为了让菌株 mh0186 生产二十二碳六烯酸（DHA），进行了罐式发酵试验。细胞的总脂肪酸含量为 466.5 mg/g 干细胞，在 26 小时时，生物量和 DHA 产量分别估计为 23.1 g/L 和 4.3 g/L。该菌株的 DHA 日产量为 4.0 g/L/d，表明我们的菌株 mh0186 较高的 DHA 生产速率应适合 DHA 的工业化生产。
