Kozlakidis Zisis, Brown Neil A, Jamal Atif, Phoon Xiyu, Coutts Robert H A
Division of Biology, Faculty of Natural Sciences, Sir Alexander Fleming Building, Imperial College London, Imperial College Road, London SW7 2AZ, UK.
Virus Genes. 2010 Feb;40(1):130-4. doi: 10.1007/s11262-009-0421-7. Epub 2009 Nov 14.
In this investigation, we show that four Phytophthora taxon douglasfir isolates from the USA, irrespective of their geographical location or host plant, and 20% of a representative cohort of Phytophthora ramorum isolates contain endornavirus dsRNAs. Three endornavirus-specific RT-PCR amplicons were generated by RT-PCR using dsRNA isolated from the four Phytophthora taxon douglasfir isolates and one representative Phytophthora ramorum isolate as template with oligonucleotide primers designed from the sequence of Phytophthora endornavirus 1. The amplified segments showed a very high degree of sequence similarity suggesting that the virus has gone through a population bottleneck during its emergence.
在本研究中，我们发现来自美国的4株樟疫霉道格拉斯冷杉分离株，无论其地理位置或寄主植物如何，以及20%的代表性群体的栎树猝死病菌分离株都含有内源病毒双链RNA。使用从4株樟疫霉道格拉斯冷杉分离株和1株代表性的栎树猝死病菌分离株中分离的双链RNA作为模板，通过逆转录聚合酶链反应（RT-PCR），使用根据樟疫霉内源病毒1的序列设计的寡核苷酸引物，产生了3个内源病毒特异性RT-PCR扩增子。扩增片段显示出非常高的序列相似性，这表明该病毒在出现过程中经历了种群瓶颈。
