Botella Leticia, Jung Marília Horta, Rost Michael, Jung Thomas
Phytophthora Research Centre, Department of Forest Protection and Wildlife Management, Faculty of Forestry and Wood Technology, Mendel University in Brno, Zemědělská 3, 613 00 Brno, Czech Republic.
Department of Genetics and Agrobiotechnology, Faculty of Agriculture and Technology, University of South Bohemia in České Budějovice, Na Sádkách 1780, 370 05 České Budějovice, Czech Republic.
J Fungi (Basel). 2022 Oct 24;8(11):1118. doi: 10.3390/jof8111118.
We explored the virome of the " complex", a group of aquatic specialists geographically limited to Southeast and East Asia, the native origin of many destructive invasive forest spp. Based on high-throughput sequencing (RNAseq) of 112 isolates of "" collected from rivers, mangroves, and ponds, and natural forests in subtropical and tropical areas in Indonesia, Taiwan, and Japan, 52 putative viruses were identified, which, to varying degrees, were phylogenetically related to the families , , , and , and the order . The prevalence of all viruses in their hosts was investigated and confirmed by RT-PCR. The rich virus composition, high abundance, and distribution discovered in our study indicate that viruses are naturally infecting taxa from the " complex" in their natural niche, and that they are predominant members of the host cellular environment. Certain Indonesian localities are the viruses' hotspots and particular "" isolates show complex multiviral infections. This study defines the first bi-segmented bunya-like virus together with the first tombus-like and botybirna-like viruses in the genus and provides insights into the spread and evolution of RNA viruses in the natural populations of an oomycete species.
我们研究了“复合体”的病毒组，这是一群在地理上局限于东南亚和东亚的水生生物，是许多具有破坏性的入侵森林物种的原生地。基于从印度尼西亚、台湾和日本亚热带和热带地区的河流、红树林、池塘及天然森林中采集的112株“”的高通量测序（RNAseq），鉴定出52种假定病毒，它们在不同程度上与 、 、 、 科以及 目在系统发育上相关。通过RT-PCR对所有病毒在其宿主中的流行情况进行了调查和确认。我们研究中发现的丰富病毒组成、高丰度和分布表明，病毒在其自然生态位中自然感染“复合体”中的分类群，并且它们是宿主细胞环境中的主要成员。某些印度尼西亚地区是病毒热点，特定的“”分离株表现出复杂的多病毒感染。本研究定义了该属中的首个双节段布尼亚样病毒以及首个番茄丛矮样病毒和番茄花叶病毒样病毒，并为RNA病毒在卵菌物种自然种群中的传播和进化提供了见解。
