流式细胞术与显微镜相比，在计数含疟色素白细胞方面更好吗？
Is flow cytometry better in counting malaria pigment-containing leukocytes compared to microscopy?
机构信息
Medical Research Unit, Hôpital Albert Schweitzer, Lambaréné, Gabon.
出版信息
Malar J. 2009 Nov 16;8:255. doi: 10.1186/1475-2875-8-255.
BACKGROUND
Detection of malaria pigment (or haemozoin; Hz)-containing leukocytes may have prognostic relevance in malaria; however, studies reported conflicting results, with microscopic counts suggestive of being inaccurate and imprecise.
METHODS
Numbers of Hz-containing leukocytes from a malaria patient obtained with a flow cytometer counting 50.000 gated events were compared with thin film microscopy as applied under field conditions.
RESULTS
Flow cytometry identified 5.8% Hz-containing monocytes and 1.8% Hz-containing neutrophils. The microscopic examination yielded 10% and 13% of Hz-containing monocytes, as well as 0% and 0.5% of Hz-containing neutrophils for observers one and two, respectively.
CONCLUSION
Novel, robust and affordable cytometric methods should be evaluated in the field as they may assist in utilizing Hz-containing cells as clinically useful parameter.
背景
检测含疟色素（或疟原血红素；Hz）的白细胞在疟疾中可能具有预后相关性；然而，已报道的研究结果相互矛盾，显微镜计数提示其不够准确和精确。
方法
使用流式细胞仪对一名疟疾患者的含 Hz 白细胞进行计数，门控事件数为 50,000 个，与现场应用的薄血膜显微镜检查进行比较。
结果
流式细胞术鉴定出 5.8%含 Hz 的单核细胞和 1.8%含 Hz 的中性粒细胞。显微镜检查分别得到 10%和 13%的含 Hz 的单核细胞，以及观察者 1 和 2 中分别为 0%和 0.5%的含 Hz 的中性粒细胞。
结论
应在现场评估新型、稳健且负担得起的细胞计量方法，因为它们可能有助于将含 Hz 细胞作为临床有用的参数进行利用。
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