Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA.
FEBS Lett. 2009 Dec 17;583(24):4019-24. doi: 10.1016/j.febslet.2009.11.029.
This paper reviews key findings from quantitative study of the yeast pheromone response system. Most come from single cell experiments that quantify molecular events the system uses to operate. After induction, signal propagation is relatively slow; peak activity takes minutes to reach the nucleus. At each measurement point along the transmission chain, signal rises, overshoots, peaks, and declines toward steady state. At at least one measurement point, this decline depends on negative feedback. The system senses and relays percent receptor occupancy, and one effect of the feedback is to maximize precision of this transmitted information. Over time, the system constantly adjusts quantitative behaviors to convey extracellular ligand concentration faithfully. These behaviors and mechanisms that control them are likely to be general for metazoan signaling systems.
本文综述了定量研究酵母信息素反应系统的关键发现。这些发现大多来自于单细胞实验，实验对系统用来运作的分子事件进行了量化。诱导后，信号传播相对较慢；达到细胞核的峰值活性需要几分钟的时间。在信号传递链的每个测量点，信号上升、过冲、峰值，然后朝着稳定状态下降。在至少一个测量点上，这种下降依赖于负反馈。该系统感知并传递受体占有率的百分比，反馈的一个作用是使传递信息的精度最大化。随着时间的推移，该系统不断调整定量行为，以忠实传递细胞外配体浓度。这些行为和控制它们的机制可能对后生动物信号系统具有普遍性。
