Eaton R W, Karns J S
Pesticide Degradation Laboratory, U.S. Department of Agriculture, Beltsville, Maryland 20705.
J Bacteriol. 1991 Feb;173(3):1363-6. doi: 10.1128/jb.173.3.1363-1366.1991.
DNA encoding the catabolism of the s-triazines ammelide and cyanuric acid was cloned from Pseudomonas sp. strain NRRLB-12228 and Klebsiella pneumoniae 99 with, as a probe, a 4.6-kb PstI fragment from a third strain, Pseudomonas sp. strain NRRLB-12227, which also encodes these activities. In strains NRRLB-12228 and 99 the ammelide aminohydrolase (trzC) and cyanuric acid amidohydrolase (trzD) genes are located on identical 4.6-kb PstI fragments which are part of a 12.4-kb DNA segment present in both strains. Strain NRRLB-12227 also carries this 12.4-kb DNA segment, except that a DNA segment of 0.8 to 1.85 kb encoding a third enzyme, ammeline aminohydrolase (trzB), has been inserted next to the ammelide aminohydrolase gene with the accompanying deletion of 1.1 to 2.15 kb of DNA. In addition, the s-triazine catabolic genes are flanked in strain NRRLB-12227 by apparently identical 2.2-kb segments that are not present in the other two strains and that seem to cause rearrangements in adjacent DNA.
编码三聚氰酸一酰胺和氰尿酸分解代谢的DNA是从假单胞菌属菌株NRRLB - 12228和肺炎克雷伯菌99中克隆得到的，以来自第三种菌株假单胞菌属菌株NRRLB - 12227的一个4.6kb PstI片段作为探针，该片段也编码这些活性。在菌株NRRLB - 12228和99中，三聚氰酸一酰胺氨基水解酶（trzC）基因和氰尿酸酰胺水解酶（trzD）基因位于相同的4.6kb PstI片段上，这些片段是两种菌株中都存在的一个12.4kb DNA片段的一部分。菌株NRRLB - 12227也携带这个12.4kb DNA片段，只是一个0.8至1.85kb编码第三种酶即三聚氰酸二酰胺氨基水解酶（trzB）的DNA片段已插入到三聚氰酸一酰胺氨基水解酶基因旁边，同时伴随有1.1至2.15kb DNA的缺失。此外，在菌株NRRLB - 12227中，三聚氰酸分解代谢基因两侧是明显相同的2.2kb片段，这两个片段在其他两种菌株中不存在，并且似乎会导致相邻DNA发生重排。
