School of Biological Sciences, University of Wollongong, Wollongong, NSW, 2522, Australia.
FASEB J. 2010 Apr;24(4):1160-6. doi: 10.1096/fj.09-144972. Epub 2009 Nov 16.
Enterohemorrhagic Escherichia coli (EHEC) strains are zoonotic pathogens responsible for a range of severe human disease. The repertoire of virulence determinants promoting EHEC disease is encoded on both the main chromosome and virulence plasmid. We examined a multiply antibiotic-resistant O26 EHEC strain for carriage of resistance genes on the virulence plasmid. The EHEC virulence plasmid containing a complex antibiotic-resistance gene locus, designated as pO26-CRL, was purified from EHEC O26:H(-) (patient with hemorrhagic colitis) and subjected to shotgun-sequencing and bioinformatic analysis. Determination of the 111,481-bp sequence of pO26-CRL revealed genes encoding a functional enterohemolysin operon (ehxCABD), STEC-specific extracellular serine protease (espP), putative EHEC adhesin (toxB), catalase/peroxidase (katP), and myristoyl transferase (msbB) involved in lipid A synthesis. A 22,609-bp Tn21 derivative is inserted within the conjugal transfer gene traC and encodes resistance to trimethoprim, streptomycin, sulfathiozole, kanamycin, neomycin, beta-lactams, and mercuric chloride. Plasmid pO26-CRL is nonconjugative but is mobilizable. This is the first report of an EHEC virulence plasmid containing a complex antibiotic resistance locus, and raises the concern that antibiotic use will coselect for virulence determinants, leading to increased disease potential in both commensal and pathogenic E. coli populations.-Venturini, C., Beatson, S. A., Djordjevic, S. P., Walker, M. J. Multiple antibiotic resistance gene recruitment onto the enterohemorrhagic Escherichia coli virulence plasmid.
肠出血性大肠杆菌（EHEC）菌株是一种人畜共患病原体，可导致多种严重人类疾病。促进 EHEC 疾病的毒力决定因素谱编码在主染色体和毒力质粒上。我们检查了一种多抗生素耐药 O26 EHEC 菌株，以研究其毒力质粒上携带的耐药基因。从 O26:H(-)（出血性结肠炎患者）EHEC 中纯化了含有复杂抗生素耐药基因座的 EHEC 毒力质粒，并对其进行了鸟枪法测序和生物信息学分析。确定 pO26-CRL 的 111481bp 序列揭示了编码功能肠溶血素操纵子（ehxCABD）、STEC 特异性细胞外丝氨酸蛋白酶（espP）、推定的 EHEC 粘附素（toxB）、过氧化氢酶/过氧化物酶（katP）和脂质 A 合成中涉及的豆蔻酰转移酶（msbB）的基因。Tn21 衍生物的 22609bp 插入在接合转移基因 traC 内，并编码对甲氧苄啶、链霉素、磺胺噻唑、卡那霉素、新霉素、β-内酰胺类和氯化汞的耐药性。pO26-CRL 质粒是非接合性的，但可移动。这是第一个报告含有复杂抗生素耐药基因座的 EHEC 毒力质粒的报告，并引起人们的关注，即抗生素的使用将共同选择毒力决定因素，从而增加共生和致病性大肠杆菌种群的疾病潜力。-Venturini，C.，Beatson，S. A.，Djordjevic，S. P.，Walker，M. J. 肠出血性大肠杆菌毒力质粒上复杂抗生素耐药基因的募集。
