Rakhimova Elza, Wiehlmann Lutz, Brauer Aimee L, Sethi Sanjay, Murphy Timothy F, Tümmler Burkhard
Klinische Forschergruppe, Abteilung für Pädiatrische Pneumologie und Neonatologie, OE 6710, Medizinische Hochschule Hannover, D-30625 Hannover, Germany.
J Infect Dis. 2009 Dec 15;200(12):1928-35. doi: 10.1086/648404.
The role played by airway infections with Pseudomonas aeruginosa in the course and pathogenesis of chronic obstructive pulmonary disease (COPD) has not yet been resolved. We report on the molecular epidemiology and population biology of P. aeruginosa in COPD.
P. aeruginosa isolates collected from adults with COPD during a 10-year prospective study were genotyped in 56 binary marker loci of core and accessory genomes.
The typing of 134 P. aeruginosa COPD isolates uncovered 60 unrelated bacterial clones. The worldwide dominant clones in the P. aeruginosa population were also the most abundant clones among the COPD isolates. Sporadic or intermittent infection with P. aeruginosa was typical for the airways of patients with COPD. Sequential isolates with the same genotype of the core genome diversified the composition of their accessory genome during the course of the infection.
Intraclonal microevolution and the frequent turnover or loss of clones are typical for infections with P. aeruginosa in COPD. This epidemiological signature differs from that of the chronic carriage of the same P. aeruginosa clone in patients with cystic fibrosis.
铜绿假单胞菌引起的气道感染在慢性阻塞性肺疾病（COPD）病程及发病机制中所起的作用尚未明确。我们报告了COPD中铜绿假单胞菌的分子流行病学及群体生物学情况。
在一项为期10年的前瞻性研究中，对从成年COPD患者分离出的铜绿假单胞菌菌株在核心基因组和辅助基因组的56个二元标记位点进行基因分型。
对134株COPD患者来源的铜绿假单胞菌菌株进行分型，发现60个不相关的细菌克隆。铜绿假单胞菌群体中的全球优势克隆也是COPD分离株中最丰富的克隆。COPD患者气道中典型的是铜绿假单胞菌的散发性或间歇性感染。在感染过程中，具有相同核心基因组基因型的连续分离株其辅助基因组的组成会发生变化。
克隆内微进化以及克隆的频繁更替或缺失是COPD中铜绿假单胞菌感染的典型特征。这种流行病学特征不同于囊性纤维化患者中相同铜绿假单胞菌克隆的慢性携带情况。
