Departamento de Microbiología II, Facultad de Farmacia, Universidad Complutense, Madrid, Spain.
Diagn Microbiol Infect Dis. 2010 Sep;68(1):20-7. doi: 10.1016/j.diagmicrobio.2010.04.008.
Pseudomonas aeruginosa is isolated in sputum cultures from cystic fibrosis (CF) patients and adults with bronchiectasis (BS) and chronic obstructive pulmonary disease, but it is not well known if the characteristics of colonization in these latter patients are similar to those with CF. We examined 125 P. aeruginosa isolates obtained from 31 patients suffering from these diseases by pulsed field gel electrophoresis and genotyping of mucA and fpvA genes. The pattern of colonization, with dominance of a clonal strain and incidence of mucoid phenotypes, was similar in every group of patients; however, in some CF and BS patients, we detected the replacement or coexistence of 2 main clones. The main differences were found in the nucleotide position of less common mucA mutations, other than mucA22, and in the predominance of the different types of the pyoverdine receptor. Our results support a similar colonization pattern by P. aeruginosa in the different obstructive pulmonary diseases.
铜绿假单胞菌从囊性纤维化（CF）患者和支气管扩张症（BS）及慢性阻塞性肺疾病（COPD）成人的痰培养中分离出来，但尚不清楚这些后一类患者的定植特征是否与 CF 患者相似。我们通过脉冲场凝胶电泳和 mucA 和 fpvA 基因的基因分型，检查了来自 31 名患有这些疾病的患者的 125 株铜绿假单胞菌分离株。定植模式具有克隆株优势和粘液表型发生率，在每一组患者中均相似；然而，在一些 CF 和 BS 患者中，我们检测到 2 种主要克隆的替代或共存。主要差异在于除 mucA22 以外的 mucA 少见突变的核苷酸位置以及不同类型的吡咯菌素受体的优势。我们的结果支持铜绿假单胞菌在不同阻塞性肺疾病中的定植模式相似。
