INRA, UMR 1131 SVQV, 68021 Colmar, France.
J Gen Virol. 2010 Mar;91(Pt 3):802-8. doi: 10.1099/vir.0.012856-0. Epub 2009 Nov 18.
Viruses in the family Luteoviridae are strictly transmitted by aphids in a non-propagative, circulative and persistent mode. Virions ingested by aphids successively cross the gut and the accessory salivary gland epithelia before being released, together with saliva, into the plant vasculature. Virion transport through aphid cells occurs by a transcytosis mechanism. This study conducted a transcriptomic analysis of intestinal genes of the pea aphid Acyrthosiphon pisum following uptake of pea enation mosaic virus. Among the 7166 transcripts analysed, 128 were significantly regulated (105 genes downregulated and 23 upregulated). Of these genes, 5 % were involved in intracellular trafficking, endocytosis and signal transduction, three important steps in the internalization and transport of virions. The limited levels of downregulation (maximum of 3.45-fold) and upregulation (maximum of 1.37-fold) suggest that the virus hijacks a constitutive endocytosis-exocytosis mechanism without heavily perturbing cell metabolism. Although limited to about 20 % of the pea aphid genes, this work represents the first large-scale analysis of aphid gene regulation following virus acquisition. A better knowledge of this virus-vector interaction will be possible only when tools representing the complete genomic capacity of the aphid become available.
家族 Luteoviridae 的病毒通过蚜虫以非增殖、循环和持久的方式严格传播。蚜虫摄入的病毒粒子依次穿过肠道和辅助唾液腺上皮细胞，然后与唾液一起释放到植物脉管系统中。病毒粒子通过转胞吞作用机制在蚜虫细胞中运输。本研究对豌豆蚜 Acyrthosiphon pisum 摄入豌豆卷叶病毒后肠道基因进行了转录组分析。在分析的 7166 个转录本中，有 128 个基因显著调控（105 个下调和 23 个上调）。在这些基因中，有 5%参与细胞内运输、内吞作用和信号转导，这是病毒内化和运输的三个重要步骤。下调的水平（最大 3.45 倍）和上调的水平（最大 1.37 倍）有限，表明病毒劫持了一种组成型的内吞作用-外排作用机制，而不会严重干扰细胞代谢。尽管这仅限于豌豆蚜基因的约 20%，但这项工作代表了在获得病毒后对蚜虫基因调控的首次大规模分析。只有当代表蚜虫完整基因组能力的工具可用时，才能更好地了解这种病毒-载体相互作用。
