Grosmaitre Xavier, Fuss Stefan H, Lee Anderson C, Adipietro Kaylin A, Matsunami Hiroaki, Mombaerts Peter, Ma Minghong
Department of Neuroscience, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA.
J Neurosci. 2009 Nov 18;29(46):14545-52. doi: 10.1523/JNEUROSCI.2752-09.2009.
The current consensus model in mammalian olfaction is that the detection of millions of odorants requires a large number of odorant receptors (ORs) and that each OR interacts selectively with a small subset of odorants, which are typically related in structure. Here, we report the odorant response properties of an OR that deviates from this model: SR1, a mouse OR that is abundantly expressed in sensory neurons of the septal organ and also of the main olfactory epithelium. Patch-clamp recordings reveal that olfactory sensory neurons (OSNs) that express SR1 respond to many, structurally unrelated odorants, and over a wide concentration range. Most OSNs expressing a gene-targeted SR1 locus that lacks the SR1 coding sequence do not show this broad responsiveness. Gene transfer in the heterologous expression system Hana3A confirms the broad response profile of SR1. There may be other mouse ORs with such broad response profiles.
目前哺乳动物嗅觉的共识模型认为，数百万种气味剂的检测需要大量的气味受体（ORs），并且每个OR选择性地与一小部分通常在结构上相关的气味剂相互作用。在此，我们报告了一种偏离该模型的OR的气味反应特性：SR1，一种在鼻中隔器官以及主要嗅觉上皮的感觉神经元中大量表达的小鼠OR。膜片钳记录显示，表达SR1的嗅觉感觉神经元（OSNs）对许多结构不相关的气味剂有反应，且反应浓度范围很广。大多数表达缺乏SR1编码序列的基因靶向SR1位点的OSNs没有表现出这种广泛的反应性。在异源表达系统Hana3A中的基因转移证实了SR1的广泛反应谱。可能还有其他具有这种广泛反应谱的小鼠ORs。
