Division of Nutrition, St. John's Research Institute, St. John's National Academy of Health Sciences, Bangalore 560034, India.
J Nutr. 2010 Jan;140(1):54-9. doi: 10.3945/jn.109.113357. Epub 2009 Nov 18.
We recently reported the lysine requirement of school-aged children living in Canada consuming a mixed diet to be 35 mg.kg(-1).d(-1). Because the majority of children in the world live on cereal-based diets in developing countries, we measured the daily lysine requirement in healthy children living in India and consuming a mostly cereal-based diet. Our objective in this study was to determine the lysine requirement in healthy, school-aged children in the developing world by using the indicator amino acid oxidation method with l-[1-(13)C] phenylalanine. Six healthy, school-aged children consumed 7 levels of lysine (5, 15, 25, 35, 50, 65, and 80 mg.kg(-1).d(-1)) each in a random order along with an amino acid mixture providing energy and protein intakes of 1.7 x resting energy expenditure and 1.5 g.kg(-1).d(-1), respectively. The mean lysine requirement was determined by applying a 2-phase linear regression crossover analysis on tracer oxidation (F(13)CO(2)) data, which identified a breakpoint (requirement) at minimal F(13)CO(2) in response to the graded lysine intakes. The mean lysine requirements with the upper 95% CI for children were determined to be 33.5 and 46.6 mg.kg(-1).d(-1), respectively, by breakpoint analysis of the F(13)CO(2) data. The mean lysine requirements of Indian children were almost identical to that of Canadian children (35 mg.kg(-1).d(-1)). There is no evidence for any adaptation in lysine requirements in children from developing countries such as India.
我们最近报道了居住在加拿大的食用混合膳食的学龄儿童的赖氨酸需要量为 35mg/kg·d。因为世界上大多数儿童在发展中国家以谷物为主食，所以我们测量了生活在印度、以谷物为主食的健康儿童的每日赖氨酸需要量。本研究的目的是通过使用 L-[1-(13)C]苯丙氨酸的指示剂氨基酸氧化法来确定发展中国家健康学龄儿童的赖氨酸需要量。6 名健康的学龄儿童以随机顺序摄入 7 个不同水平的赖氨酸（5、15、25、35、50、65 和 80mg/kg·d），同时摄入氨基酸混合物，以提供能量和蛋白质摄入量分别为休息能量消耗的 1.7 倍和 1.5g/kg·d。通过对示踪剂氧化（F(13)CO(2))数据进行 2 相线性回归交叉分析，确定了最小 F(13)CO(2)的转折点（需求），从而确定了赖氨酸的需求。通过对 F(13)CO(2)数据的转折点分析，确定了儿童的平均赖氨酸需求分别为 33.5 和 46.6mg/kg·d，置信区间上限为 95%。通过对 F(13)CO(2)数据的转折点分析，确定了印度儿童的平均赖氨酸需求分别为 33.5 和 46.6mg/kg·d，置信区间上限为 95%。印度等发展中国家的儿童的赖氨酸需求没有证据表明存在适应性变化。
