1类整合子中该基因的表达
Expression of the Gene in Class 1 Integrons.
作者信息
Raherison Sophie, Jove Thomas, Gaschet Margaux, Pinault Emilie, Tabesse Aurore, Torres Carmen, Ploy Marie-Cécile
机构信息
University of Limoges, UMR_S1092, Limoges, France
INSERM, U1092, Limoges, France.
出版信息
Antimicrob Agents Chemother. 2017 Apr 24;61(5). doi: 10.1128/AAC.02704-16. Print 2017 May.
Abstract
is a plasmid-mediated quinolone resistance gene embedded within a gene cassette, most often within an integron. It confers resistance to quinolones and aminoglycosides. We investigated the role of a 101-bp fragment frequently present upstream of the gene cassette and found that it contributes to the expression of and provides an alternative start codon, confirming the length of the AAC(6')-Ib-cr protein to 199 amino acids.
摘要
是一个嵌入基因盒内的质粒介导喹诺酮抗性基因，最常见于整合子中。它赋予对喹诺酮类和氨基糖苷类的抗性。我们研究了基因盒上游经常出现的一个101 bp片段的作用，发现它有助于的表达并提供一个替代起始密码子，证实了AAC(6')-Ib-cr蛋白的长度为199个氨基酸。
相似文献
1
Expression of the Gene in Class 1 Integrons.1类整合子中该基因的表达
Antimicrob Agents Chemother. 2017 Apr 24;61(5). doi: 10.1128/AAC.02704-16. Print 2017 May.
2
High prevalence of plasmid-mediated quinolone resistance genes qnr and aac(6')-Ib-cr in clinical isolates of Enterobacteriaceae from nine teaching hospitals in China.中国九家教学医院临床分离的肠杆菌科细菌中质粒介导喹诺酮耐药基因qnr和aac(6')-Ib-cr的高流行率
Antimicrob Agents Chemother. 2008 Dec;52(12):4268-73. doi: 10.1128/AAC.00830-08. Epub 2008 Sep 22.
3
High prevalence of plasmid-mediated quinolone resistance determinants qnr, aac(6')-Ib-cr, and qepA among ceftiofur-resistant Enterobacteriaceae isolates from companion and food-producing animals.在来自伴侣动物和食用动物的耐头孢噻呋肠杆菌科分离株中，质粒介导的喹诺酮耐药决定子qnr、aac(6')-Ib-cr和qepA的高流行率。
Antimicrob Agents Chemother. 2009 Feb;53(2):519-24. doi: 10.1128/AAC.00886-08. Epub 2008 Oct 20.
4
Appearance of aac(6')-Ib-cr gene among extended-spectrum beta-lactamase-producing Enterobacteriaceae in a French hospital.法国一家医院产超广谱β-内酰胺酶肠杆菌科细菌中aac(6')-Ib-cr基因的出现情况
J Infect. 2008 Jun;56(6):454-9. doi: 10.1016/j.jinf.2008.03.010. Epub 2008 Apr 28.
5
Differential distribution of plasmid-mediated quinolone resistance genes in clinical enterobacteria with unusual phenotypes of quinolone susceptibility from Argentina.阿根廷具有异常表型的临床肠杆菌中，质粒介导的喹诺酮耐药基因的差异分布。
Antimicrob Agents Chemother. 2013 Jun;57(6):2467-75. doi: 10.1128/AAC.01615-12. Epub 2013 Mar 11.
6
Plasmid mediated quinolone resistance determinants qnr, aac(6')-Ib-cr, and qep in ESBL-producing Escherichia coli clinical isolates from Egypt.埃及产超广谱β-内酰胺酶大肠埃希菌临床分离株中质粒介导的喹诺酮耐药决定簇qnr、aac(6')-Ib-cr和qep
Indian J Med Microbiol. 2012 Oct-Dec;30(4):442-7. doi: 10.4103/0255-0857.103766.
7
Analysis of plasmid-mediated multidrug resistance in Escherichia coli and Klebsiella oxytoca isolates from clinical specimens in Japan.日本临床标本中分离的大肠杆菌和产酸克雷伯菌的质粒介导多重耐药性分析。
Int J Antimicrob Agents. 2009 Oct;34(4):347-50. doi: 10.1016/j.ijantimicag.2009.05.007. Epub 2009 Jun 27.
8
Prevalence of plasmid-mediated quinolone resistance genes in uropathogenic Escherichia coli isolated in a teaching hospital of northern Italy.意大利北部一所教学医院分离的尿路致病性大肠埃希菌中质粒介导的喹诺酮耐药基因的流行情况。
Microb Drug Resist. 2012 Feb;18(1):33-41. doi: 10.1089/mdr.2010.0146. Epub 2011 Jun 28.
9
Surveillance for plasmid-mediated quinolone resistance determinants in Enterobacteriaceae within the Calgary Health Region, Canada: the emergence of aac(6')-Ib-cr.加拿大卡尔加里健康区域内肠杆菌科细菌中质粒介导喹诺酮耐药决定子的监测：aac(6')-Ib-cr的出现
J Antimicrob Chemother. 2008 May;61(5):999-1002. doi: 10.1093/jac/dkn068. Epub 2008 Feb 21.
10
Is plasmid-mediated quinolone resistance a clinically significant problem?质粒介导的喹诺酮耐药性是一个具有临床意义的问题吗？
Clin Microbiol Infect. 2008 Apr;14(4):295-7. doi: 10.1111/j.1469-0691.2007.01930.x. Epub 2008 Jan 7.
引用本文的文献
1
Shifts in genes and Class 1 integron prevalence in beta-lactamase-producing bacteria before and after the COVID-19 pandemic in Mendoza, Argentina.阿根廷门多萨新冠疫情前后产β-内酰胺酶细菌中基因和1类整合子流行率的变化。
Microbiol Spectr. 2025 Aug 5;13(8):e0277124. doi: 10.1128/spectrum.02771-24. Epub 2025 Jul 15.
2
Three simple and cost-effective assays for AAC(6')-Ib-cr enzyme activity.三种用于检测AAC(6')-Ib-cr酶活性的简单且经济高效的检测方法。
Front Microbiol. 2025 Apr 25;16:1513425. doi: 10.3389/fmicb.2025.1513425. eCollection 2025.
3
First Report of and Variant Genes Associated with Mutations in Encoded Fluoroquinolone Resistance in Avian Strains Collected in Tunisia.**中文译文**：突尼斯采集的禽源 株中与编码氟喹诺酮类药物耐药性的 基因突变相关的 和 变异基因的首次报告。
Int J Mol Sci. 2023 Nov 9;24(22):16116. doi: 10.3390/ijms242216116.
4
Integron Functionality and Genome Innovation: An Update on the Subtle and Smart Strategy of Integrase and Gene Cassette Expression Regulation.整合子功能与基因组创新：整合酶及基因盒表达调控的精妙策略新进展
Microorganisms. 2022 Jan 20;10(2):224. doi: 10.3390/microorganisms10020224.
5
Genetic and Biochemical Characterization of OXA-519, a Novel OXA-48-Like β-Lactamase.OXA-519，一种新型 OXA-48 类β-内酰胺酶的遗传和生化特性研究。
Antimicrob Agents Chemother. 2018 Jul 27;62(8). doi: 10.1128/AAC.00469-18. Print 2018 Aug.
6
Characterization of the novel In1059 harbouring VIM gene cassette.携带VIM基因盒的新型In1059的特征分析。
Antimicrob Resist Infect Control. 2017 May 18;6:50. doi: 10.1186/s13756-017-0204-1. eCollection 2017.
本文引用的文献
1
Rise and dissemination of aminoglycoside resistance: the aac(6')-Ib paradigm.氨基糖苷类耐药性的兴起和传播：aac(6')-Ib 范例。
Front Microbiol. 2013 May 17;4:121. doi: 10.3389/fmicb.2013.00121. eCollection 2013.
2
Intrahospitalary dissemination of Klebsiella pneumoniae carrying bla(DHA-1) and qnrB4 genes within a novel complex class 1 integron.携带bla(DHA-1)和qnrB4基因的肺炎克雷伯菌在新型1类复合整合子内的医院内传播
Diagn Microbiol Infect Dis. 2012 Jun;73(2):210-1. doi: 10.1016/j.diagmicrobio.2012.02.011. Epub 2012 Mar 24.
3
qnr, aac(6')-Ib-cr and qepA genes in Escherichia coli and Klebsiella spp.: genetic environments and plasmid and chromosomal location.qnr、aac(6')-Ib-cr 和 qepA 基因在大肠杆菌和克雷伯氏菌属中的研究：遗传环境以及质粒和染色体定位。
J Antimicrob Chemother. 2012 Apr;67(4):886-97. doi: 10.1093/jac/dkr548. Epub 2012 Jan 4.
4
Inverse correlation between promoter strength and excision activity in class 1 integrons.在 class 1 整合子中，启动子强度与切除活性呈负相关。
PLoS Genet. 2010 Jan;6(1):e1000793. doi: 10.1371/journal.pgen.1000793. Epub 2010 Jan 8.
5
Characterization of two novel gene cassettes, dfrA27 and aadA16, in a non-O1, non-O139 Vibrio cholerae isolate from China.从中国的一株非 O1、非 O139 霍乱弧菌分离株中鉴定出两个新的基因盒 dfrA27 和 aadA16。
Clin Microbiol Infect. 2010 Aug;16(8):1125-9. doi: 10.1111/j.1469-0691.2009.03060.x. Epub 2009 Nov 10.
6
Gene cassettes and cassette arrays in mobile resistance integrons.移动性抗性整合子中的基因盒及盒阵列
FEMS Microbiol Rev. 2009 Jul;33(4):757-84. doi: 10.1111/j.1574-6976.2009.00175.x. Epub 2009 Apr 15.
7
Complex class 1 integrons with diverse variable regions, including aac(6')-Ib-cr, and a novel allele, qnrB10, associated with ISCR1 in clinical enterobacterial isolates from Argentina.来自阿根廷临床肠杆菌分离株中具有不同可变区的复杂1类整合子，包括aac(6')-Ib-cr和一个与ISCR1相关的新等位基因qnrB10。
Antimicrob Agents Chemother. 2007 Dec;51(12):4466-70. doi: 10.1128/AAC.00726-07. Epub 2007 Oct 15.
8
Fluoroquinolone-modifying enzyme: a new adaptation of a common aminoglycoside acetyltransferase.氟喹诺酮修饰酶：一种常见氨基糖苷类乙酰转移酶的新适应性变体
Nat Med. 2006 Jan;12(1):83-8. doi: 10.1038/nm1347. Epub 2005 Dec 20.
9
Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.使用实时定量PCR和2(-ΔΔC(T))方法分析相对基因表达数据。
Methods. 2001 Dec;25(4):402-8. doi: 10.1006/meth.2001.1262.
10
Characterization of Class 1 integrons from Pseudomonas aeruginosa that contain the bla(VIM-2) carbapenem-hydrolyzing beta-lactamase gene and of two novel aminoglycoside resistance gene cassettes.对含有bla(VIM-2)碳青霉烯水解β-内酰胺酶基因的铜绿假单胞菌1类整合子以及两个新型氨基糖苷类耐药基因盒的鉴定。
Antimicrob Agents Chemother. 2001 Feb;45(2):546-52. doi: 10.1128/AAC.45.2.546-552.2001.
Zotero 插件