Suganuma Masatoshi, Teh Aik Hong, Makino Masatomo, Shimizu Nobutaka, Kaneko Tomonori, Hirata Kunio, Yamamoto Masaki, Kumasaka Takashi
Department of Life Science, Tokyo Institute of Technology, Japan.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 Nov 1;65(Pt 11):1128-30. doi: 10.1107/S1744309109038846. Epub 2009 Oct 30.
RsbX from Bacillus subtilis is a manganese-dependent PPM phosphatase and negatively regulates the signal transduction of the general stress response by the dephosphorylation of RsbS and RsbR, which are activators of the alternative RNA polymerase sigma factor SigB. In order to elucidate the structural-functional relationship of its Ser/Thr protein-phosphorylation mechanism, an X-ray crystallographic diffraction study of RsbX was performed. Recombinant RsbX was expressed in Escherichia coli, purified and crystallized. Crystals were obtained using the sitting-drop vapour-diffusion method and X-ray diffraction data were collected to 1.06 angstrom resolution with an R(merge) of 8.1%. The crystals belonged to the triclinic space group P1, with unit-cell parameters a = 33.3, b = 41.7, c = 68.6 angstrom , alpha = 98.8, beta = 90.0, gamma = 108.4 degrees.
来自枯草芽孢杆菌的RsbX是一种依赖锰的PPM磷酸酶，它通过使RsbS和RsbR去磷酸化来负向调节一般应激反应的信号转导，RsbS和RsbR是替代RNA聚合酶西格玛因子SigB的激活剂。为了阐明其丝氨酸/苏氨酸蛋白磷酸化机制的结构-功能关系，对RsbX进行了X射线晶体学衍射研究。重组RsbX在大肠杆菌中表达、纯化并结晶。采用坐滴气相扩散法获得晶体，并收集了分辨率为1.06埃、合并R值为8.1%的X射线衍射数据。晶体属于三斜晶系空间群P1，晶胞参数为a = 33.3、b = 41.7、c = 68.6埃，α = 98.8、β = 90.0、γ = 108.4°。
