Laboratoire de Biochimie et de Génie Enzymatique des Lipases, ENIS route de soukra, BP1173-3038 Sfax, Tunisia.
Appl Biochem Biotechnol. 2010 Oct;162(3):662-70. doi: 10.1007/s12010-009-8849-8. Epub 2009 Nov 19.
The aim of the present work was to evaluate the anti-inflammatory properties of Cynara cardunculus L. (Asteraceae) during its growth using various solvents such as n-hexane, dichloromethane, acetone, and methanol for air-dried leaves and stems. The anti-inflammatory activities of crude extracts were evaluated by measuring the inhibition potency of mammalian non-pancreatic phospholipases A2 (hG-IIA). The methanol and acetone extracts of leaves harvested in February exhibit potent inhibition of hG-IIA (IC(50) = 50 and 70 microg/ml, respectively). However, the acetone extract of stems harvested in December inhibits the hG-IIA with a lower IC(50) around 130 microg/ml. Fractionation on silica gel and hydrophobic gel of the methanol extract of leaves harvested in February increases the inhibitory effect, and the IC(50) reached 10 microg/ml.
本工作旨在评估不同溶剂（正己烷、二氯甲烷、丙酮和甲醇）提取的羽衣甘蓝（菊科）在其生长过程中的抗炎特性，所用材料为风干的叶片和茎。通过测量对哺乳动物非胰腺磷脂酶 A2（hG-IIA）的抑制能力来评估粗提取物的抗炎活性。2 月收获的羽衣甘蓝叶片的甲醇和丙酮提取物对 hG-IIA 具有很强的抑制作用（IC50 分别为 50 和 70μg/ml）。然而，12 月收获的茎的丙酮提取物对 hG-IIA 的抑制作用较弱，IC50 约为 130μg/ml。2 月收获的叶片甲醇提取物经硅胶和疏水凝胶柱层析分离后，抑制作用增强，IC50 达到 10μg/ml。
