Center for Biotechnology and Microbiology University of Peshawar, Peshawar, Pakistan.
Biotechnol J. 2010 Nov;5(11):1207-15. doi: 10.1002/biot.201000020.
A new compound, 6a,11a-dihydro-6H-[1] benzofuro [3,2-c][1,3]dioxolo[4,5-g]chromen-9-ol was isolated from the ethyl acetate fraction of Vitex agnus-castus. The structure of this compound was identified with the help of spectroscopic techniques ((13)C NMR, (1)H NMR, HMBC, HMQC, NOESY and COSY). The compound showed low urease- (32.0%) and chymotrypsin- (31.4%) inhibitory activity, and moderate (41.3%) anti-inflammatory activity. The crude extract and various fractions obtained from the aerial parts of the plant were also screened for possible in vitro hemagglutination, antibacterial and phytotoxic activities. No hemagglutination activity against human erythrocytes was observed in crude extracts and fractions of V. agnus-castus. The fractions and crude methanolic extract showed moderate and low antibacterial activity. Exceptions were the CHCl(3) fraction, which showed significant antibacterial activity against Klebsiella pneumonia (81% with MIC(50)=2.19 mg/mL), the n-hexane fraction, which exhibited no activity against Salmonella typhi, and the CHCl(3) and aqueous fractions, which showed no activity against Bacillus pumalis. Moderate phytotoxic activity (62.5%) was observed by n-hexane fraction of V. agnus-castus against Lemna minor L at 1000 μg/mL.
从牡荆属植物乙酸乙酯部分中分离得到一种新的化合物，6a,11a-二氢-6H-[1]苯并呋喃[3,2-c][1,3]二氧杂环戊[4,5-g]色烯-9-醇。该化合物的结构通过光谱技术（(13)C NMR、(1)H NMR、HMBC、HMQC、NOESY 和 COSY）确定。该化合物表现出低的脲酶（32.0%）和胰凝乳蛋白酶（31.4%）抑制活性，以及中等（41.3%）抗炎活性。还对植物地上部分的粗提取物和各种馏分进行了体外血红细胞凝集、抗菌和植物毒性活性筛选。在牡荆属植物的粗提取物和馏分中未观察到对人红细胞的血红细胞凝集活性。馏分和粗甲醇提取物表现出中等和低的抗菌活性。例外的是 CHCl(3)馏分，对肺炎克雷伯菌（81%，MIC(50)=2.19 mg/mL）具有显著的抗菌活性，正己烷馏分对伤寒沙门氏菌无活性，而 CHCl(3)和水馏分对巨大芽孢杆菌无活性。牡荆属植物的正己烷馏分在 1000 μg/mL 时对浮萍（Lemna minor L）表现出中等的植物毒性活性（62.5%）。
