Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, Tokyo, Japan.
J Mater Sci Mater Med. 2010 Apr;21(4):1263-72. doi: 10.1007/s10856-009-3938-3. Epub 2009 Nov 19.
The hydroxyapatite/collagen (HAp/Col) sponge with 95% (v/v) porosity was prepared by freeze-drying of a HAp/Col fiber suspension. MG63 cells were seeded onto the HAp/Col sponge and cultured under a pressure/perfusion condition with osteogenic supplements. A collagen (Col) sponge was used as a control. The cells with sponge were examined by a histology, total DNA content and gene expression. The cells showed good attachment and proliferation everywhere in the HAp/Col sponge, while the cells mainly proliferated at the peripheral part of the Col sponge. Thus, total DNA content in the HAp/Col sponges reached 1.8 times greater than that in the Col sponges at Day 21. Further, the cells and extracellular matrix only in the HAp/Col sponge were calcified, although the cells in both sponge evenly expressed osteogenic gene. These results suggest that the HAp/Col sponge could be useful as a scaffold for bone tissue engineering.
通过冷冻干燥 HAp/Col 纤维悬浮液制备了具有 95%（体积/体积）孔隙率的羟基磷灰石/胶原（HAp/Col）海绵。将 MG63 细胞接种到 HAp/Col 海绵上，并在具有成骨补充剂的压力/灌注条件下进行培养。使用胶原（Col）海绵作为对照。通过组织学、总 DNA 含量和基因表达检查带有海绵的细胞。细胞在 HAp/Col 海绵的各处都表现出良好的附着和增殖，而细胞主要在 Col 海绵的外周部分增殖。因此，在第 21 天，HAp/Col 海绵中的总 DNA 含量比 Col 海绵中的总 DNA 含量高 1.8 倍。此外，只有在 HAp/Col 海绵中的细胞和细胞外基质才钙化，尽管两种海绵中的细胞都均匀地表达成骨基因。这些结果表明，HAp/Col 海绵可用作骨组织工程的支架。
