Swedish Medical Center, Trauma Research, Englewood, CO, USA.
Scand J Trauma Resusc Emerg Med. 2009 Nov 19;17:57. doi: 10.1186/1757-7241-17-57.
In critical injury, the occurrence of increased oxidative stress or a reduced antioxidant status has been observed. The purpose of this study was to correlate the degree of oxidative stress, by measuring the oxidation-reduction potential (ORP) of plasma in the critically injured, with injury severity and serum amyloid A (SAA) levels.
A total of 140 subjects were included in this retrospective study comprising 3 groups: healthy volunteers (N = 21), mild to moderate trauma (ISS < 16, N = 41), and severe trauma (ISS >or= 16, N = 78). For the trauma groups, plasma was collected on an almost daily basis during the course of hospitalization. ORP analysis was performed using a microelectrode, and ORP maxima were recorded for the trauma groups. SAA, a sensitive marker of inflammation in critical injury, was measured by liquid chromatography/mass spectrometry.
ORP maxima were reached on day 3 (+/- 0.4 SEM) and day 5 (+/- 0.5 SEM) for the ISS < 16 and ISS >or= 16 groups, respectively. ORP maxima were significantly higher in the ISS < 16 (-14.5 mV +/- 2.5 SEM) and ISS >or= 16 groups (-1.1 mV +/- 2.3 SEM) compared to controls (-34.2 mV +/- 2.6 SEM). Also, ORP maxima were significantly different between the trauma groups. SAA was significantly elevated in the ISS >or= 16 group on the ORP maxima day compared to controls and the ISS < 16 group.
The results suggest the presence of an oxidative environment in the plasma of the critically injured as measured by ORP. More importantly, ORP can differentiate the degree of oxidative stress based on the severity of the trauma and degree of inflammation.
在严重创伤中，已经观察到氧化应激增加或抗氧化状态降低。本研究的目的是通过测量严重创伤患者血浆的氧化还原电位（ORP），将氧化应激程度与损伤严重程度和血清淀粉样蛋白 A（SAA）水平相关联。
本回顾性研究共纳入 140 例受试者，分为 3 组：健康志愿者（N=21）、轻度至中度创伤（ISS<16，N=41）和严重创伤（ISS≥16，N=78）。对于创伤组，在住院期间几乎每天采集血浆。使用微电极进行 ORP 分析，并记录创伤组的 ORP 最大值。SAA 是严重创伤中炎症的敏感标志物，通过液相色谱/质谱法进行测量。
ISS<16 组和 ISS≥16 组的 ORP 最大值分别在第 3 天（+/-0.4 SEM）和第 5 天（+/-0.5 SEM）达到。ISS<16 组（-14.5 mV +/- 2.5 SEM）和 ISS≥16 组（-1.1 mV +/- 2.3 SEM）的 ORP 最大值明显高于对照组（-34.2 mV +/- 2.6 SEM）。此外，ISS<16 组和 ISS≥16 组之间的 ORP 最大值也存在显著差异。与对照组和 ISS<16 组相比，ISS≥16 组在 ORP 最大值日的 SAA 显著升高。
这些结果表明，严重创伤患者的血浆中存在氧化环境，可通过 ORP 测量。更重要的是，ORP 可以根据创伤的严重程度和炎症程度来区分氧化应激的程度。
