a Department of Surgery , Larner College of Medicine, University of Vermont , Burlington , VT , USA.
Redox Rep. 2018 Dec;23(1):125-129. doi: 10.1080/13510002.2018.1456000.
Oxidation-reduction potential (ORP) measurement can demonstrate the extent of oxidative stress in patients with severe illness and/or injury. A novel ORP diagnostic platform using disposable sensors (RedoxSYS) has been validated by comparison to mass spectrometry, but the optimal methods of sample handling for best performance of the device have not been described.
We sought to optimize ORP measurement in human plasma under controlled conditions. We hypothesized that the anticoagulant, freeze-thawing, and storage duration would influence measured ORP levels.
The platform was sensitive to exogenous oxidation with hydrogen peroxide and reduction with ascorbic acid. Plasma anticoagulated with heparin was more sensitive to differences in ORP than plasma prepared in citrate. ORP measurements decreased slightly after a freeze-thaw cycle, but once frozen, ORP was stable for up to one month.
We confirm that ORP detects oxidative stress in plasma samples. Optimal measurement of plasma ORP requires blood collection in heparin anticoagulant tubes and immediate analysis without a freeze-thaw cycle.
氧化还原电位 (ORP) 测量可显示重症疾病和/或损伤患者的氧化应激程度。一种新型的使用一次性传感器的 ORP 诊断平台（RedoxSYS）已经通过与质谱的比较得到了验证，但为了获得设备的最佳性能，尚未描述最佳的样本处理方法。
我们试图在受控条件下优化人血浆中的 ORP 测量。我们假设抗凝剂、冻融和储存时间会影响测量的 ORP 水平。
该平台对外源氧化（过氧化氢）和还原（抗坏血酸）很敏感。与用柠檬酸盐制备的血浆相比，用肝素抗凝的血浆对 ORP 差异更敏感。冻融循环后 ORP 略有下降，但一旦冷冻，ORP 在一个月内保持稳定。
我们证实 ORP 可检测血浆样本中的氧化应激。最佳的血浆 ORP 测量需要使用肝素抗凝管采集血液，并在不进行冻融循环的情况下立即进行分析。
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