Takeda K, Kawai S, Tetsuka T, Konno K
Biochim Biophys Acta. 1977 Oct 13;484(2):301-6. doi: 10.1016/0005-2744(77)90086-9.
Prolyl hydroxylase (proline,2-oxoglutarate dioxygenase, EC 1.14.11.2) of soluble fraction (105 000 X g supernatant) of rat granulation tissues was markedly enhanced by addition of nucleoside triphosphates to the assay medium. But the stimulatory activities of nucleoside triphosphates were very different in fractions derived from tissues of rat. In skin, lung or whole fetal tissues other than granuloma, GTP enhanced the enzymatic activity by 3-4 fold. On the other hand, in kidney, liver and spleen tissues it brought about no enhancement. The same results were obtained even if ATP regenerating system was added in the assay medium. The stimulatory effect of nucleoside triphosphates was not seen with the soluble fraction of liver, but it appeared with the enzyme fraction purified by affinity column chromatography. The same phenomenon was observed by addition of bovine serum albumin instead of nucleoside triphosphates as stimulator. We discuss the possible reasons as to why the responses of the enzyme to stimulators were quite different among various tissues.
大鼠肉芽组织可溶性部分（105000×g 上清液）中的脯氨酰羟化酶（脯氨酸、2-氧代戊二酸双加氧酶，EC 1.14.11.2）在测定介质中添加核苷三磷酸后活性显著增强。但核苷三磷酸的刺激活性在源自大鼠不同组织的部分中差异很大。在皮肤、肺或除肉芽肿外的整个胎儿组织中，GTP 可使酶活性增强 3 至 4 倍。另一方面，在肾脏、肝脏和脾脏组织中，它并未带来活性增强。即使在测定介质中添加了 ATP 再生系统，也得到了相同的结果。核苷三磷酸对肝脏可溶性部分没有刺激作用，但对通过亲和柱色谱法纯化的酶部分有刺激作用。用牛血清白蛋白代替核苷三磷酸作为刺激剂时也观察到了相同的现象。我们讨论了该酶对刺激剂的反应在不同组织间差异很大的可能原因。
