氧化锌纳米粒子的测定、表征及对 HELF 细胞的细胞毒性。
Determination, characterization and cytotoxicity on HELF cells of ZnO nanoparticles.
机构信息
Nanjing Centers for Disease Control and Prevention, Nanjing, PR China.
出版信息
Colloids Surf B Biointerfaces. 2010 Mar 1;76(1):145-50. doi: 10.1016/j.colsurfb.2009.10.028. Epub 2009 Oct 27.
Three kinds of ZnO nanoparticles (NPs) with different size were characterized with transmission electron microscopy (TEM), X-ray diffraction (XRD). The potential cytotoxicity of ZnO NPs with various concentrations has been investigated using human embryonic lung fibroblasts (HELF) cells. The cytotoxicity of ZnO NPs on the normal HELF cell was evaluated by 3-(4,5-dimethylthiazol)-2,5-diphenltetrazoliumhromide (MTT) assay and characterized with photo microscopy and scanning electron microscopy (SEM). The results indicated that ZnO NPs aqueous suspensions with concentrations varying from 2.5 to 150mgL(-1) were harmful to the HELF cells and the cytotoxicity to HELF showed obviously concentration dependence. With concentrations higher than 20mgL(-1), ZnO NPs led to the livability of the tested cells lower than 10%. These results highlighted the need for caution during the use of ZnO NPs above 1.2-1.6mgL(-1) for safety. Finally, an UV-vis spectroscopic approach was developed for the determination of ZnO NPs concentration in the linear range 2-50mgL(-1) (correlation coefficients more than 0.9990), and the detection limit was 1.0mgL(-1).
三种不同尺寸的氧化锌纳米粒子（NPs）通过透射电子显微镜（TEM）和 X 射线衍射（XRD）进行了表征。使用人胚肺成纤维细胞（HELF）细胞研究了不同浓度的 ZnO NPs 的潜在细胞毒性。通过 3-（4,5-二甲基噻唑）-2,5-二苯基四氮唑溴化物（MTT）测定法评估了 ZnO NPs 对正常 HELF 细胞的细胞毒性，并通过相差显微镜和扫描电子显微镜（SEM）进行了表征。结果表明，浓度范围为 2.5 至 150mgL(-1) 的 ZnO NPs 水悬浮液对 HELF 细胞有害，且 HELF 的细胞毒性表现出明显的浓度依赖性。浓度高于 20mgL(-1)时，ZnO NPs 导致测试细胞的存活率低于 10%。这些结果强调了在使用 ZnO NPs 时需要谨慎，安全浓度应低于 1.2-1.6mgL(-1)。最后，开发了一种紫外可见分光光度法用于测定 2-50mgL(-1) 范围内 ZnO NPs 的浓度（相关系数大于 0.9990），检测限为 1.0mgL(-1)。
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