Pop Daniel, Buzatu Roxana, Moacă Elena-Alina, Watz Claudia Geanina, Cîntă-Pînzaru Simona, Barbu Tudoran Lucian, Nekvapil Fran, Avram Ștefana, Dehelean Cristina Adriana, Crețu Marius Octavian, Nicolov Mirela, Szuhanek Camelia, Jivănescu Anca
Department of Prosthodontics, Faculty of Dental Medicine, "Victor Babes" University of Medicine and Pharmacy, Revolutiei Ave. 1989, No. 9, RO-300580 Timișoara, Romania.
TADERP Reseach Center-Advanced and Digital Techniques for Endodontic, Restorative and Prosthetic Treatment, "Victor Babeș" University of Medicine and Pharmacy, Revolutiei Ave. 1989, No. 9, RO-300041 Timişoara, Romania.
Materials (Basel). 2021 Jun 25;14(13):3556. doi: 10.3390/ma14133556.
The current study presents the effect of naked FeO@Carbon nanoparticles obtained by the combustion method on primary human gingival fibroblasts (HGFs) and primary gingival keratinocytes (PGKs)-relevant cell lines of buccal oral mucosa. In this regard, the objectives of this study were as follows: (i) development via combustion method and characterization of nanosized magnetite particles with carbon on their surface, (ii) biocompatibility assessment of the obtained magnetic nanoparticles on HGF and PGK cell lines and (iii) evaluation of possible irritative reaction of FeO@Carbon nanoparticles on the highly vascularized chorioallantoic membrane of a chick embryo. Physicochemical properties of FeO@Carbon nanoparticles were characterized in terms of phase composition, chemical structure, and polymorphic and molecular interactions of the chemical bonds within the nanomaterial, magnetic measurements, ultrastructure, morphology, and elemental composition. The X-ray diffraction analysis revealed the formation of magnetite as phase pure without any other secondary phases, and Raman spectroscopy exhibit that the pre-formed magnetic nanoparticles were covered with carbon film, resulting from the synthesis method employed. Scanning electron microscopy shown that nanoparticles obtained were uniformly distributed, with a nearly spherical shape with sizes at the nanometric level; iron, oxygen, and carbon were the only elements detected. While biological screening of FeO@Carbon nanoparticles revealed no significant cytotoxic potential on the HGF and PGK cell lines, a slight sign of irritation was observed on a limited area on the chorioallantoic membrane of the chick embryo.
本研究展示了通过燃烧法制备的裸FeO@碳纳米颗粒对原代人牙龈成纤维细胞（HGFs）和原代牙龈角质形成细胞（PGKs）——口腔颊黏膜相关细胞系的影响。在这方面，本研究的目标如下：（i）通过燃烧法制备并表征表面带有碳的纳米级磁铁矿颗粒，（ii）评估所获得的磁性纳米颗粒对HGF和PGK细胞系的生物相容性，以及（iii）评估FeO@碳纳米颗粒对鸡胚高度血管化的绒毛尿囊膜可能产生的刺激反应。从相组成、化学结构、纳米材料内化学键的多晶型和分子相互作用、磁性测量、超微结构、形态和元素组成等方面对FeO@碳纳米颗粒的物理化学性质进行了表征。X射线衍射分析表明形成了纯相磁铁矿，没有任何其他次生相，拉曼光谱显示预先形成的磁性纳米颗粒被碳膜覆盖，这是由所采用的合成方法导致的。扫描电子显微镜显示所获得的纳米颗粒均匀分布，呈近球形，尺寸处于纳米级别；检测到的元素只有铁、氧和碳。虽然对FeO@碳纳米颗粒的生物学筛选显示其对HGF和PGK细胞系没有显著的细胞毒性潜力，但在鸡胚绒毛尿囊膜的有限区域观察到了轻微的刺激迹象。
