Canadian Institute of Health Research Team in Aboriginal Anti-diabetic Medicines, Department of Pharmacology, Université de Montréal, QC, Canada.
Phytother Res. 2010 May;24(5):741-7. doi: 10.1002/ptr.3025.
Like many aboriginal populations, First Nations communities such as the Cree of Eeyou Istchee are facing continuously increasing rates of diabetes and related complications. Advanced glycation endproducts (AGEs), which readily form and accumulate with sustained hyperglycemia, contribute to the development of diabetic complications and, as such, are considered a potential therapeutic target. In the present study, the inhibition of AGE formation by ethanolic extracts of the Cree medicinal plant Vaccinium vitis-idaea L. was assessed by fluorometric detection of fluorescent AGEs and immunodetection of N(epsilon)-(carboxymethyl)lysine adducts of albumin. Extracts from V. vitis-idaea berries demonstrated a concentration-dependent inhibition of AGE formation in both measures. High performance liquid chromatography mass spectrometry (HPLC/MS) identified nine main phenolic constituents. Four were selected for further testing, of which catechin, quercetin-3-O-galactoside and cyanidin-3-O-glucoside but not para-coumaric acid displayed antiglycation activities. These results demonstrate that the flavonoid components of the berry extract are potent antiglycation agents and provide pharmacological validation for the traditional use of V. vitis-idaea as an antidiabetic remedy.
像许多原住民群体一样，克里族等第一民族社区（如魁北克的伊努伊特人）正面临着糖尿病及其相关并发症发病率不断上升的问题。糖基化终产物（AGEs）在持续高血糖的情况下很容易形成并积累，会导致糖尿病并发症的发生，因此被认为是一种潜在的治疗靶点。在本研究中，通过荧光法检测荧光 AGEs 和免疫法检测白蛋白的 N(epsilon)-(羧甲基)赖氨酸加合物，评估了克里药用植物蔓越莓 Vaccinium vitis-idaea L. 的乙醇提取物对 AGE 形成的抑制作用。V. vitis-idaea 浆果的提取物在这两种测量方法中均表现出浓度依赖性的 AGE 形成抑制作用。高效液相色谱-质谱联用（HPLC/MS）鉴定出了 9 种主要的酚类成分。选择其中 4 种进行进一步测试，结果表明儿茶素、槲皮素-3-O-半乳糖苷和矢车菊素-3-O-葡萄糖苷而非对香豆酸具有抗糖化活性。这些结果表明，该浆果提取物的类黄酮成分是有效的抗糖化剂，为蔓越莓作为抗糖尿病药物的传统用途提供了药理学验证。
