Chen Q, Li D Y, Shitaka Y, Oiwa K
Department of Biomedical Engineering, University of Alberta, Edmonton, Alberta, T6G 2V2, Canada.
J Nanosci Nanotechnol. 2009 Sep;9(9):5123-33. doi: 10.1166/jnn.2009.1204.
In vitro motility assays, where protein motors (attached to a surface) move protein filaments, have been used for investigating protein motors' functions. In recent decades, these assays are extended to explore potential applications of motor proteins as biological motors in nano-bio-machine development. Recent attempts include fabricating micrometer-scale tracks on the surface to confine and guide the flow of bio-filaments as a power transfer medium driven by the motor proteins. Understanding the interaction between bio-filaments and fabricated tracks as well as the mutual interaction between bio-filaments is of importance to the design of potential nano-bio-machines. In this study, we investigate the behaviors of a microtubule driven by axonemal dynein at the collision against another microtubule and micro-fabricated walls, respectively. Based on experimental observations, we propose a model to study possible mechanisms for the microtubule-microtubule and microtubule-wall interactions, which involve bumping force, bending moment and torque generation.
体外运动性测定，即让蛋白质马达（附着在表面）移动蛋白质细丝，已被用于研究蛋白质马达的功能。近几十年来，这些测定被扩展到探索马达蛋白作为生物马达在纳米生物机器开发中的潜在应用。最近的尝试包括在表面制造微米级轨道，以限制和引导生物细丝的流动，作为由马达蛋白驱动的动力传递介质。了解生物细丝与制造的轨道之间的相互作用以及生物细丝之间的相互作用对于潜在纳米生物机器的设计至关重要。在本研究中，我们分别研究了轴丝动力蛋白驱动的微管与另一微管和微加工壁碰撞时的行为。基于实验观察，我们提出了一个模型来研究微管-微管和微管-壁相互作用的可能机制，其中涉及碰撞力、弯矩和扭矩的产生。
