Institute of Neuroscience, State Key Laboratory of Neuroscience, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
Sleep. 2009 Nov;32(11):1417-24. doi: 10.1093/sleep/32.11.1417.
Sleep is crucial to memory consolidation in humans and other animals; however, the effect of insufficient sleep on subsequent learning and memory remains largely elusive.
Learning and memory after 1-day sleep deprivation (slpD) was evaluated using Pavlovian olfactory conditioning in Drosophila, and locomotor activity was measured using the Drosophila Activity Monitoring System in a 12:12 light-dark cycle.
We found that slpD specifically impaired 1-h memory in wild type Canton-S flies, and this effect could persist for at least 2 h. However, alternative stresses (heat stress, oxidative stress, starvation, and rotation stress) did not result in a similar effect and left the flies' memory intact. Mechanistic studies demonstrated that flies with either silenced transmission of the mushroom body (MB) during slpD or down-regulated cAMP levels in the MB demonstrated no slpD-induced 1-h memory impairment.
We found that slpD specifically impaired 1-h memory in Drosophila, and either silencing of MB transmission during slpD or down-regulation of the cAMP level in the MB protected the flies from slpD-induced impairment.
睡眠对人类和其他动物的记忆巩固至关重要；然而，睡眠不足对随后的学习和记忆的影响在很大程度上仍难以捉摸。
使用果蝇的巴甫洛夫嗅觉条件反射来评估 1 天睡眠剥夺（slpD）后的学习和记忆，并用果蝇活动监测系统在 12:12 光照-黑暗周期下测量运动活动。
我们发现 slpD 特异性地损害了野生型 Canton-S 果蝇的 1 小时记忆，这种影响至少可以持续 2 小时。然而，其他应激源（热应激、氧化应激、饥饿和旋转应激）并没有产生类似的效果，并且保持了果蝇的记忆完整。机制研究表明，在 slpD 期间沉默蘑菇体（MB）的传递或降低 MB 中的 cAMP 水平的果蝇没有表现出 slpD 诱导的 1 小时记忆损伤。
我们发现 slpD 特异性地损害了果蝇的 1 小时记忆，在 slpD 期间沉默 MB 传递或降低 MB 中的 cAMP 水平可保护果蝇免受 slpD 诱导的损伤。
