Future Scientists' School, Shizuoka University, Oya 836, Shizuoka, 422-8529, Japan.
Research Institute of Green Science and Technology, Shizuoka University, Oya 836, Shizuoka, 422-8529, Japan.
Sci Rep. 2021 Apr 30;11(1):9395. doi: 10.1038/s41598-021-88967-1.
The importance of sleep in maintaining cognitive functions such as learning and memory has been reported in both vertebrates and invertebrates. Previous studies demonstrated that sleep deprivation impaired the olfactory memory retention of fruit flies as described in the classical conditioning paradigm. Here, we show that sleep deprivation leads to a preference for the odours of the rearing environment in Drosophila melanogaster. Flies whose sleep had been disturbed with periodic rotation stimuli during night-time preferred apple cider vinegar (ACV) to broth, while this preference was lower in flies without sleep deprivation and those rotated during daytime. Experiments using single odours showed an increase in responses to ACV due to sleep deprivation. These results suggest that sleep functions in food odour preference. Flies grown on medium supplemented with ACV showed greater preference for ACV, and those grown with broth supplementation showed a greater preference for broth under sleep-deprived conditions. These results suggest that flies with night-time sleep deprivation become attached to the environment on which they have developed, and that sleep contributes to preference for novel food odours. This study offers an approach to investigating the interaction between sleep and neural disorders concerning cognitive deficits towards novel stimuli.
睡眠对学习和记忆等认知功能的重要性在脊椎动物和无脊椎动物中都有报道。以前的研究表明，睡眠剥夺会损害果蝇的嗅觉记忆保留，正如经典条件作用范式所描述的那样。在这里，我们表明睡眠剥夺会导致果蝇对饲养环境气味的偏好。在夜间受到周期性旋转刺激干扰睡眠的果蝇更喜欢苹果醋（ACV）而不是肉汤，而在没有睡眠剥夺和白天旋转的果蝇中，这种偏好较低。使用单一气味的实验表明，由于睡眠剥夺，对 ACV 的反应增加。这些结果表明睡眠在食物气味偏好中起作用。在补充有 ACV 的培养基中生长的果蝇对 ACV 的偏好更大，而在补充有肉汤的培养基中生长的果蝇在睡眠剥夺条件下对肉汤的偏好更大。这些结果表明，夜间睡眠剥夺的果蝇会依恋它们所生长的环境，而睡眠有助于对新食物气味的偏好。这项研究提供了一种方法来研究睡眠与涉及对新刺激认知缺陷的神经紊乱之间的相互作用。
