Department of Kinesiology, The University of Texas at Austin , Austin, TX, USA.
Tissue Eng Part A. 2010 Apr;16(4):1395-405. doi: 10.1089/ten.TEA.2009.0226.
The loss of a portion of skeletal muscle poses a unique challenge for the normal regeneration of muscle tissue. A transection injury with tissue loss will not heal due to the gap between muscle segments. A damage model was developed by removing a portion of the lateral gastrocnemius (GAS) of Sprague-Dawley rats. Maximal isometric, tetanic tension (P(o)) was measured after the removal of either a small defect (0.5 x 1.0 cm) or a large defect (1.0 x 1.0 cm) piece of the GAS. In situ P(o) immediately after creation of the defect was 88.3 +/- 2.0% of the nonoperated contralateral GAS force for small defect and 76.9 +/- 3.2% of control for large defect. No functional recovery occurred in either group over the course of 28 days. To enhance recovery, a homologous, decellularized, muscle extracellular matrix (ECM) was implanted into the 1 x 1 cm defect of the lateral GAS of Lewis rats. After 42 days, growth of blood vessels and myofibers into the ECM was apparent, but no restoration of P(o) occurred. These data demonstrate the ability of the ECM to support muscle and blood vessel regeneration, but full recovery of function does not occur after 42 days.
骨骼肌的部分丧失给肌肉组织的正常再生带来了独特的挑战。由于肌肉段之间存在间隙，组织丢失的横断损伤不会愈合。通过切除 Sprague-Dawley 大鼠外侧比目鱼肌（GAS）的一部分来开发损伤模型。在切除 0.5 x 1.0 cm 或 1.0 x 1.0 cm 大小的 GAS 小块后，测量最大等长、强直张力（Po）。在小缺陷（0.5 x 1.0 cm）或大缺陷（1.0 x 1.0 cm）形成后立即进行原位 Po，小缺陷的未操作对侧 GAS 力的 88.3 +/- 2.0%，大缺陷的 76.9 +/- 3.2%。在 28 天的过程中，两组均未发生功能恢复。为了增强恢复能力，将同源的脱细胞肌肉细胞外基质（ECM）植入 Lewis 大鼠外侧 GAS 的 1 x 1 cm 缺陷处。42 天后，明显看到血管和肌纤维生长到 ECM 中，但 Po 没有恢复。这些数据表明 ECM 能够支持肌肉和血管再生，但在 42 天后功能没有完全恢复。
