Tan Yong How, Helms Haylie R, Nakayama Karina H
Department of Biomedical Engineering, Oregon Health and Science University, Portland, OR, United States.
Front Bioeng Biotechnol. 2022 Feb 28;10:831300. doi: 10.3389/fbioe.2022.831300. eCollection 2022.
Cardiovascular disease is the leading cause of death worldwide and is associated with approximately 17.9 million deaths each year. Musculoskeletal conditions affect more than 1.71 billion people globally and are the leading cause of disability. These two areas represent a massive global health burden that is perpetuated by a lack of functionally restorative treatment options. The fields of regenerative medicine and tissue engineering offer great promise for the development of therapies to repair damaged or diseased tissues. Decellularized tissues and extracellular matrices are cornerstones of regenerative biomaterials and have been used clinically for decades and many have received FDA approval. In this review, we first discuss and compare methods used to produce decellularized tissues and ECMs from cardiac and skeletal muscle. We take a focused look at how different biophysical properties such as spatial topography, extracellular matrix composition, and mechanical characteristics influence cell behavior and function in the context of regenerative medicine. Lastly, we describe emerging research and forecast the future high impact applications of decellularized cardiac and skeletal muscle that will drive novel and effective regenerative therapies.
心血管疾病是全球首要死因，每年约有1790万人与之相关。肌肉骨骼疾病影响着全球超过17.1亿人，是导致残疾的主要原因。这两个领域构成了巨大的全球健康负担，由于缺乏功能性修复治疗方案，这种负担持续存在。再生医学和组织工程领域为开发修复受损或患病组织的疗法带来了巨大希望。脱细胞组织和细胞外基质是再生生物材料的基石，已在临床上使用了数十年，许多已获得美国食品药品监督管理局（FDA）的批准。在本综述中，我们首先讨论并比较从心脏和骨骼肌中制备脱细胞组织和细胞外基质的方法。我们重点关注不同的生物物理特性，如空间拓扑结构、细胞外基质组成和力学特性，如何在再生医学背景下影响细胞行为和功能。最后，我们描述新兴研究，并预测脱细胞心脏和骨骼肌未来的高影响力应用，这些应用将推动新颖有效的再生疗法。
