Department of Pathology, Faculty of Veterinary Medicine, University of São Paulo, São Paulo, SP, Brazil.
Vector Borne Zoonotic Dis. 2010 Jun;10(5):481-7. doi: 10.1089/vbz.2008.0089.
The ingestion of food products originating from poultry infected with Salmonella spp. is one of the major causes of food poisoning in humans. The control of poultry salmonellosis is particularly difficult since birds are asymptomatic and numerous factors may expedite the maintenance of bacteria in poultry production facilities.
The aim of the study was to determine the vectorial capacity of adults and larvae of Alphitobius diaperinus (Coleoptera: Tenebrionidae) in the experimental transmission of Salmonella Enteritidis phage type 4 to 1-day-old specific pathogen-free White Leghorn chicks.
Adult insects and larvae were starved for 1 day, fed for 24 h or 7 days on sterile ration that had been treated with Salmonella Enteritidis phage type 4, and the levels of bacterial infection were determined. Infected adult insects and larvae were fed to groups of day-old chicks, after which bacteria were recovered from cecum, liver, and spleen samples over a 7-day period.
Infected larvae were more efficient than adult insects in transmitting Salmonella Enteritidis to chicks. Higher concentrations of bacteria could be reisolated from the cecum, liver, and spleen of chicks that had ingested infected larvae compared with those that had ingested infected adults.
The control of A. diaperinus, and particularly of the larvae, represents a critical factor in the reduction of Salmonella spp. in poultry farms.
人类因食用受沙门氏菌属污染的禽产品而中毒是主要原因之一。由于禽类无症状且有许多因素可加速其在禽类生产设施中的细菌存活，因此控制禽沙门氏菌病特别困难。
本研究的目的是确定黄粉虫成虫和幼虫（鞘翅目：拟步行虫科）在实验性传播肠炎沙门氏菌噬菌体 4 型给 1 日龄无菌白来航鸡雏中的媒介能力。
成虫和幼虫饥饿 1 天，然后在无菌饲料上喂养 24 小时或 7 天，该饲料已用肠炎沙门氏菌噬菌体 4 型处理，并确定细菌感染水平。将受感染的成虫和幼虫喂给 1 日龄雏鸡组，然后在 7 天内从盲肠、肝脏和脾脏样本中回收细菌。
感染的幼虫比成虫更有效地将肠炎沙门氏菌传播给雏鸡。与食用感染成虫的雏鸡相比，食用感染幼虫的雏鸡盲肠、肝脏和脾脏中可重新分离出更高浓度的细菌。
控制黄粉虫，特别是幼虫，是减少禽场中沙门氏菌属的关键因素。
