Department of Drug Delivery Technology, Leiden University , Leiden, The Netherlands.
Tissue Eng Part A. 2010 Apr;16(4):1433-41. doi: 10.1089/ten.TEA.2009.0358.
In this study we generated human skin equivalents (HSEs) under submerged conditions mimicking the aqueous in utero environment and investigated the morphology and differentiation process of the formed epidermis. Further, the skin barrier, which resides in the stratum corneum (SC), was characterized by its lipid content, hydration level, and natural moisturizing factor level. The submerged HSEs showed comparable tissue morphology and similar expression of several differentiation markers and SC lipid composition compared with HSEs grown at the air-liquid interface and native human skin. The SC of the submerged HSEs, however, contained more free water and less natural moisturizing factors compared with the air-exposed counterparts. These results show that the presented cell culture method can be utilized to generate HSEs under submerged conditions to study epidermal formation under aqueous conditions.
在这项研究中，我们在模拟羊水内环境的水下条件下生成了人皮肤等效物（HSE），并研究了形成的表皮的形态和分化过程。此外，位于角质层（SC）中的皮肤屏障的特性是其脂质含量、水合水平和天然保湿因子水平。与在气液界面和天然人体皮肤中生长的 HSE 相比，水下 HSE 显示出可比的组织形态和类似的几种分化标志物和 SC 脂质组成的表达。然而，与暴露于空气中的对应物相比，水下 HSE 的 SC 含有更多的游离水和更少的天然保湿因子。这些结果表明，所提出的细胞培养方法可用于在水下条件下生成 HSE，以研究水相条件下的表皮形成。
