Sales Mariana Lázaro, Fonseca Antônio Augusto, Sales Erica Bravo, Cottorello Ana Cláudia Pinto, Issa Marina Azevedo, Hodon Mikael Arrais, Soares Filho Paulo Martins, Ramalho Alberto Knust, Silva Marcio Roberto, Lage Andrey Pereira, Heinemann Marcos Bryan
Lanagro/MG, Pedro Leopoldo, Minas Gerais, Brazil.
Folia Microbiol (Praha). 2014 Sep;59(5):433-8. doi: 10.1007/s12223-014-0317-3. Epub 2014 Apr 18.
Mycobacterium tuberculosis complex (MTC) comprises a group of bacteria that have a high degree of genetic similarity. Two species in this group, Mycobacterium tuberculosis and Mycobacterium bovis, are the main cause of human and bovine tuberculosis, respectively. M. bovis has a broader host range that includes humans; thus, the differentiation of mycobacterium is of great importance for epidemiological and public health considerations and to optimize treatment. The current study aimed to evaluate primers and molecular markers described in the literature to differentiate M. bovis and M. tuberculosis by PCR. Primers JB21/22, frequently cited in scientific literature, presented in our study the highest number of errors to identify M. bovis or M. tuberculosis (73%) and primers Mb.400, designed to flank region of difference 4 (RD4), were considered the most efficient (detected all M. bovis tested and did not detect any M. tuberculosis tested). Although also designed to flank RD4, primers Mb.115 misidentified eight samples due to primer design problems. The results showed that RD4 is the ideal region to differentiate M. bovis from other bacteria classified in MTC, but primer design should be considered carefully.
结核分枝杆菌复合群（MTC）由一组具有高度遗传相似性的细菌组成。该组中的两个物种，结核分枝杆菌和牛分枝杆菌，分别是人类和牛结核病的主要病因。牛分枝杆菌的宿主范围更广，包括人类；因此，分枝杆菌的鉴别对于流行病学和公共卫生考量以及优化治疗非常重要。当前的研究旨在评估文献中描述的用于通过聚合酶链反应（PCR）鉴别牛分枝杆菌和结核分枝杆菌的引物和分子标记。科学文献中经常引用的引物JB21/22在我们的研究中鉴别牛分枝杆菌或结核分枝杆菌时出现错误的数量最多（73%），而设计用于差异区域4（RD4）侧翼区域的引物Mb.400被认为是最有效的（检测出了所有测试的牛分枝杆菌，且未检测出任何测试的结核分枝杆菌）。尽管引物Mb.115也是设计用于RD4侧翼区域，但由于引物设计问题，错误鉴定了8个样本。结果表明，RD4是将牛分枝杆菌与MTC中分类的其他细菌区分开来的理想区域，但引物设计应仔细考虑。
