Adachi H, Degawa M, Miura S, Hashimoto Y, Sugimura T, Esumi H
Biochemistry Division, National Cancer Center Research Institute, Tokyo, Japan.
Biochem Biophys Res Commun. 1991 Jan 31;174(2):797-803. doi: 10.1016/0006-291x(91)91488-x.
Changes in hepatic enzymes responsible for mutagenic activation of food mutagens-carcinogens by treatment with 2-amino-1-methyl-6-phenylimidazo [4, 5-b]pyridine (PhIP) in male F344 rats were examined using the Salmonella mutation test, with 3 heterocyclic aromatic amines as substrates, and further characterized by Western blot analysis with anti-P450 monoclonal antibodies (MoAbs) against rat P450IA1 and P450IA2. Enzymatical and immunochemical analyses indicated that PhIP could induce a putative new P450 isozyme, mol. wt., 51,000, together with P450IA1 and P450IA2 in rat liver microsomes. The profiles of induced P450 molecular species varied dramatically, depending on the time after PhIP administration.
利用沙门氏菌突变试验，以3种杂环芳香胺为底物，检测用2-氨基-1-甲基-6-苯基咪唑并[4,5-b]吡啶（PhIP）处理雄性F344大鼠后，负责食物诱变剂-致癌物诱变激活的肝酶变化，并通过用抗大鼠P450IA1和P450IA2的抗P450单克隆抗体（MoAbs）进行蛋白质印迹分析进一步表征。酶学和免疫化学分析表明，PhIP可在大鼠肝微粒体中诱导一种假定的新P450同工酶，分子量为51,000，同时诱导P450IA1和P450IA2。诱导的P450分子种类的谱根据PhIP给药后的时间而有显著变化。
