Degawa M, Tanimura S, Agatsuma T, Hashimoto Y
Department of Hygienic Chemistry, Pharmaceutical Institute, Tohoku University, Sendai, Japan.
Carcinogenesis. 1989 Jun;10(6):1119-22. doi: 10.1093/carcin/10.6.1119.
Male F344 rats were treated with hepatocarcinogenic heterocyclic aromatic amines such as amino acid- and protein-pyrolysate components (Trp P-1, Trp P-2, Glu P-1, Glu P-2, A alpha C, MeA alpha C, IQ and MeIQx) and changes in microsomal cytochrome P-450 isozymes in the livers were examined by means of immuno-Western blotting using anti-rat cytochrome P-450 monoclonal antibodies. The results suggested that all chemicals tested induce cytochrome P-448 isozymes, particularly cytochrome P-448H (P-450IA2), which efficiently mediate mutagenic activation of the carcinogens. This was substantiated by the enzymatic analyses with the substrates showing different characters to rat cytochrome P-450 isozyme-mediated mutagenesis.
雄性F344大鼠用致癌性杂环芳香胺如氨基酸和蛋白质热解产物成分（Trp P - 1、Trp P - 2、Glu P - 1、Glu P - 2、AαC、MeAαC、IQ和MeIQx）进行处理，通过使用抗大鼠细胞色素P - 450单克隆抗体的免疫蛋白质印迹法检测肝脏微粒体细胞色素P - 450同工酶的变化。结果表明，所有测试的化学物质均诱导细胞色素P - 448同工酶，特别是细胞色素P - 448H（P - 450IA2），其能有效介导致癌物的诱变活化。用对大鼠细胞色素P - 450同工酶介导的诱变具有不同特性的底物进行酶分析证实了这一点。
