Degawa M, Kobayashi K, Miura S, Arai H, Esumi H, Sugimura T, Hashimoto Y
Department of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Tohoku University, Sendai.
Jpn J Cancer Res. 1992 Oct;83(10):1047-51. doi: 10.1111/j.1349-7006.1992.tb02720.x.
Rats, mice, hamsters and guinea pigs were given an i.p. injection of 2-amino-1-methyl-6-phenyl-imidazo[4,5-b]pyridine (PhIP), a protein-derived pyrolysate component present in cooked foods, and inductions of cytochrome P450 (P450) in the liver and kidney of these animals were examined. The activity and amount of P450s corresponding to the rat P450IA1 and P450IA2 were assessed by means of a bacterial mutation test using 3 carcinogenic heterocyclic aromatic amines including PhIP as substrates and by Western blotting with a monoclonal antibody reactive with both P450IA1 and P450IA2. In rats, PhIP induced P450IA1, P450IA2 and a new but unspecified P450 isozyme in the liver, and induced P450IA1 in the kidney. However, PhIP induced none of these P450 isozymes in mice, hamsters and guinea pigs.
给大鼠、小鼠、仓鼠和豚鼠腹腔注射2-氨基-1-甲基-6-苯基咪唑并[4,5-b]吡啶（PhIP），它是一种存在于熟食中的蛋白质衍生热解产物成分，然后检测这些动物肝脏和肾脏中细胞色素P450（P450）的诱导情况。使用包括PhIP在内的3种致癌杂环芳香胺作为底物，通过细菌突变试验以及用与P450IA1和P450IA2都反应的单克隆抗体进行蛋白质印迹法，评估与大鼠P450IA1和P450IA2相对应的P450的活性和含量。在大鼠中，PhIP诱导肝脏中的P450IA1、P450IA2和一种新的但未明确的P450同工酶，并诱导肾脏中的P450IA1。然而，PhIP在小鼠、仓鼠和豚鼠中未诱导出这些P450同工酶中的任何一种。
