State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai 200237, People's Republic of China.
J Ind Microbiol Biotechnol. 2010 Aug;37(8):857-62. doi: 10.1007/s10295-010-0733-6. Epub 2010 May 14.
Serrawettin W1 produced by Serratia marcescens is a surface-active exolipid resulting in a lot foam formation during the 2,3-butanediol (2,3-BD) fermentation process. In order to avoid excessive addition of antifoam agent and microbial contamination, S. marcescens mutants deficient in serrawettin W1 formation were successfully constructed through insertional inactivation of the swrW gene coding for serrawettin W1 synthase. The shake flask and batch experiments suggested that disruption of the swrW gene led to significant reduction of the foam formation and improved 2,3-BD production a little. Ultimately, fed-batch culturing of the mutant afforded a maximum 2,3-BD concentration of 152 g l(-1) with a productivity of 2.67 g l(-1) h(-1) and a yield of 92.6% at 57 h.
粘质沙雷氏菌产生的表面活性外脂肽 Serrawettin W1 在 2,3-丁二醇（2,3-BD）发酵过程中会导致大量泡沫形成。为了避免过度添加消泡剂和微生物污染，通过插入失活编码 Serrawettin W1 合酶的 swrW 基因，成功构建了粘质沙雷氏菌突变体，该突变体缺乏 Serrawettin W1 的形成。摇瓶和分批实验表明，swrW 基因的破坏导致泡沫形成显著减少，2,3-BD 产量略有提高。最终，该突变株的补料分批培养在 57 h 时获得了 152 g/L 的最大 2,3-BD 浓度，产率为 2.67 g/L/h，产率为 92.6%。
