T盒机制：作为调控分子的转运RNA
The T box mechanism: tRNA as a regulatory molecule.
作者信息
Green Nicholas J, Grundy Frank J, Henkin Tina M
机构信息
Department of Microbiology, Center for RNA Biology, The Ohio State University, Columbus, OH 43210, USA.
出版信息
FEBS Lett. 2010 Jan 21;584(2):318-24. doi: 10.1016/j.febslet.2009.11.056.
Abstract
The T box mechanism is widely used in Gram-positive bacteria to regulate expression of aminoacyl-tRNA synthetase genes and genes involved in amino acid biosynthesis and uptake. Binding of a specific uncharged tRNA to a riboswitch element in the nascent transcript causes a structural change in the transcript that promotes expression of the downstream coding sequence. In most cases, this occurs by stabilization of an antiterminator element that competes with formation of a terminator helix. Specific tRNA recognition by the nascent transcript results in increased expression of genes important for tRNA aminoacylation in response to decreased pools of charged tRNA.
摘要
T 盒机制广泛应用于革兰氏阳性菌中，以调控氨酰-tRNA 合成酶基因以及参与氨基酸生物合成和摄取的基因的表达。特定的无负载 tRNA 与新生转录本中的核糖开关元件结合，会导致转录本发生结构变化，从而促进下游编码序列的表达。在大多数情况下，这是通过稳定抗终止子元件来实现的，该元件可与终止子螺旋的形成相互竞争。新生转录本对特定 tRNA 的识别会导致在负载 tRNA 库减少时，对 tRNA 氨酰化很重要的基因的表达增加。
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