Institute of Mechanics, Chinese Academy of Sciences, Beijing, China.
Biosens Bioelectron. 2010 Feb 15;25(6):1530-4. doi: 10.1016/j.bios.2009.10.030. Epub 2009 Oct 30.
A novel method is reported for the detection of avian influenza virus subtype H5 using a biosensor based on high spatial resolution imaging ellipsometry (IE). Monoclonal antibodies specific to H5 hemagglutinin protein were immobilized on silicon wafers and used to capture virus particles. Resultant changes on the surface of the wafers were visualized directly in gray-scale on an imaging ellipsometry image. This preliminary study has shown that the assay is rapid and specific for the identification of avian influenza virus subtype H5. Compared with lateral-flow immunoassays, this biosensor not only has better sensitivity, but can also simultaneously perform multiplexed tests. These results suggest that this biosensor might be a valuable diagnostic tool for avian influenza virus detection.
报道了一种使用基于高空间分辨率成像椭圆光度法（IE）的生物传感器检测甲型 H5 流感病毒的新方法。将针对 H5 血凝素蛋白的单克隆抗体固定在硅晶片上，并用于捕获病毒颗粒。在成像椭圆光度图像上，可以直接以灰度级显示晶片表面上的变化。这项初步研究表明，该测定法快速且专一地用于鉴定甲型 H5 流感病毒。与侧向流动免疫测定法相比，这种生物传感器不仅具有更高的灵敏度，而且还可以同时进行多重测试。这些结果表明，该生物传感器可能是一种用于检测禽流感病毒的有价值的诊断工具。
