Niu Yu, Kang Tengfei, Jin Gang
NML, Institute of Mechanics, Chinese Academy of Sciences, 15 Bei-si-huan West Road, Beijing 100190, China.
University of Chinese Academy of Sciences, 19A Yu-quan Road, Beijing 100049, China.
Thin Solid Films. 2014 Nov 28;571:453-462. doi: 10.1016/j.tsf.2014.01.043. Epub 2014 Jan 24.
Tumor marker detection contributes to the early diagnosis of cancers. However, due to the lack of detection specificity, its results cannot act as a direct evidence to confirm cancer occurrence in clinic. Joint detection of tumor markers may improve the detection specificity. As a trial for clinical diagnosis of hepatocellular carcinoma, α-fetoprotein, α-l-fucosidase and ferritin have been combined and detected with a label-free, phase sensitive and high throughput imaging ellipsometry biosensor (IEB). Eighty-two sera have been quantitatively detected with IEB and the results are in agreement with the clinical standard approaches. Evaluated by receiver operating characteristic analysis, the specificity of joint detection improves remarkably with IEB for hepatocellular carcinoma. It can be foreseen that the joint detection of tumor markers with IEB has a potential for clinical cancer diagnosis.
肿瘤标志物检测有助于癌症的早期诊断。然而，由于缺乏检测特异性，其结果在临床上不能作为确诊癌症发生的直接证据。联合检测肿瘤标志物可能会提高检测特异性。作为肝细胞癌临床诊断的一项试验，甲胎蛋白、α-L-岩藻糖苷酶和铁蛋白已被结合起来，并用一种无标记、相敏且高通量成像椭圆偏振生物传感器（IEB）进行检测。已用IEB对82份血清进行了定量检测，结果与临床标准方法一致。通过受试者工作特征分析评估，使用IEB联合检测肝细胞癌的特异性显著提高。可以预见，用IEB联合检测肿瘤标志物在临床癌症诊断方面具有潜力。
